Cytotoxic cobalt (III) Schiff base complexes: in vitro anti-proliferative, oxidative stress and gene expression studies in human breast and lung cancer cells.

Autor: Gowdhami B; Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India.; National Centre for Alternatives to Animal Experiments, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India., Manojkumar Y; Department of Chemistry, Bishop Heber College, Tiruchirappalli, Tamilnadu, India., Vimala RTV; Department of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India., Ramya V; Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India., Karthiyayini B; National Centre for Alternatives to Animal Experiments, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India.; Department of Microbiology, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India., Kadalmani B; Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India. kadalmani@bdu.ac.in., Akbarsha MA; Mahatma Gandhi-Doerenkamp Centre for Alternatives, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India. akbarbdu@gmail.com.; Department of Biotechnology & Research Coordinator, National College (Autonomous), Tiruchirappalli, Tamilnadu, 620001, India. akbarbdu@gmail.com.
Jazyk: angličtina
Zdroj: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine [Biometals] 2022 Feb; Vol. 35 (1), pp. 67-85. Date of Electronic Publication: 2021 Dec 22.
DOI: 10.1007/s10534-021-00351-8
Abstrakt: Increasing cancer drug chemo-resistance, especially in the treatment of breast and lung cancers, alarms the immediate need of newer and effective anticancer drugs. Until now, chemotherapeutics based on metal complexes are considered the most effective treatment modality. In the present study, we have evaluated the cytotoxic effect of two cobalt (III) Schiff base complexes based on the leads from complex combinatorial chemistry. Cobalt (III) Schiff base complexes (Complex 3 = Co(Ph-acacen)(HA) 2 ](ClO 4 ) and Complex 4 =  [Co(Ph-acacen)(DA) 2 ](ClO 4 )] (Ph-acacen, 1-phenylbutane-1,3-dione; DA, dodecyl amine; HA, heptylamine) were evaluated against human breast cancer cell MCF-7 and lung cancer cell A549 using MTT cell viability assay, cellular morphological changes studied by Acridine Orange and Ethidium Bromide (AO/EB), Dual fluorescent staining, Hoechst staining 33248, Comet assay, Annexin V-Cy3 and 6 CFDA assay, JC-1 staining, Reactive oxygen species (ROS) assay, Immunofluorescence assay, and Real-time reverse transcription-polymerase chain reaction (RT-qPCR). Treatment of cobalt (III) Schiff base complexes (Complex 3 & 4) affected the viability of the cancer cells. The cell death induced by the complexes was predominantly apoptosis, but necrosis also occurred to a certain extent. Complex 4 produced better cytotoxic effect than complex 3, and MCF-7 cell was more responsive than A549. In that order, the complexes were more selective to cancer cell than normal cell, and more effective in overall performance than the standard drug cisplatin. Therefore, we conclude that cobalt (III) Schiff base complexes, especially complex 4, have the potential to be developed as effective drugs for treatment of cancers in general, and breast and lung cancers in particular.
(© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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