First report on the molecular detection of Entamoeba bovis from the endangered wild water buffalo (Bubalus arnee) in Nepal.

Autor: Aryal M; Central Department of Biotechnology, Tribhuvan University, Kathmandu, Nepal., Adhikari RB; Third Pole Conservancy, Bhaktapur, Nepal., Kandel P; Kathmandu University, Dhulikhel, Nepal., Ghimire TR; Department of Zoology, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal., Khadka D; Nepal Academy of Science and Technology, Lalitpur, Nepal., Maharjan J; Nepal Academy of Science and Technology, Lalitpur, Nepal., Gaire KP; Kathmandu, Nepal., Shrestha S; Sustainable Development Initiative Centre, Kathmandu, Nepal., Manandhar KD; Central Department of Biotechnology, Tribhuvan University, Kathmandu, Nepal., Kandel RC; Department of National Parks and Wildlife Conservation, Ministry of Forests and Environment, Kathmandu, Nepal., Poudel RC; Nepal Academy of Science and Technology, Lalitpur, Nepal., Pandey K; Central Department of Zoology, Tribhuvan University, Kirtipur, Nepal.
Jazyk: angličtina
Zdroj: Veterinary medicine and science [Vet Med Sci] 2022 Mar; Vol. 8 (2), pp. 799-807. Date of Electronic Publication: 2021 Dec 17.
DOI: 10.1002/vms3.697
Abstrakt: Background: The Asiatic wild water buffalo (Bubalus arnee) is an endangered species that is conserved in the Koshi Tappu Wildlife Reserve (KTWR), Nepal, and was recently translocated to the Chitwan National Park (CNP). Gastrointestinal (GI) parasites are the cause of significant negative health and production impacts on animals worldwide.
Methods: A coprological survey of GI parasites of wild water buffalo was carried out in the CNP in 2020. Fresh dung samples (n = 25) were collected from wild water buffaloes and analysed using sedimentation and flotation techniques for morphological identification of parasite cysts, oocysts and eggs.
Results: Nine different GI parasites were recorded of which Entamoeba spp. (20 samples, 80%) were the most common. The presence of Entamoeba spp. was further validated using polymerase chain reaction (PCR) analysis and DNA sequencing. The PCR results were positive for all of the microscopically positive samples, and the species was identified as Entamoeba bovis. Three samples were sequenced and formed a cluster of E. bovis, which was separated from other Entamoeba spp. in phylogenetic analysis.
Conclusion: This is the first report for molecular detection of E. bovis from wild water buffaloes in Nepal. Future work should focus on the prevalence of such infections in water buffaloes in forest environments.
(© 2021 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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