Processing and Analysis of Ascites.
| Autor: | Micek HM; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA., Carroll MJ; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA., Barroilhet L; Department of Obstetrics and Gynecology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.; University of Wisconsin Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA., Kreeger PK; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. kreeger@wisc.edu.; Department of Cell and Regenerative Biology, Department of Obstetrics and Gynecology, and University of Wisconsin Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA. kreeger@wisc.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2424, pp. 95-104. |
| DOI: | 10.1007/978-1-0716-1956-8_5 |
| Abstrakt: | The accumulation of peritoneal fluid, referred to as ascites, is common in ovarian cancer. This fluid is a complex mixture that may include cells as well as a diverse array of cytokines and growth factors. Here we describe a comprehensive method to process ascites to maximize data collection. The cellular fraction and fluid are first separated by centrifugation. The fluid can be frozen for later analysis of soluble factors or for use in in vitro experiments. The cellular fraction can be processed to analyze its composition or stored for future use. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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