Visual-stimuli Four-arm Maze test to Assess Cognition and Vision in Mice.

Autor: Vit JP; Department of Neurosurgery, Maxine Dunitz Neurosurgical Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA., Fuchs DT; Department of Neurosurgery, Maxine Dunitz Neurosurgical Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA., Angel A; Pharmaseed Ltd., Ness Ziona 74047, Israel., Levy A; Pharmaseed Ltd., Ness Ziona 74047, Israel., Lamensdorf I; Pharmaseed Ltd., Ness Ziona 74047, Israel., Black KL; Department of Neurosurgery, Maxine Dunitz Neurosurgical Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA., Koronyo Y; Department of Neurosurgery, Maxine Dunitz Neurosurgical Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA., Koronyo-Hamaoui M; Department of Neurosurgery, Maxine Dunitz Neurosurgical Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA.; Department of Biomedical Sciences, Division of Applied Cell Biology and Physiology, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Jazyk: angličtina
Zdroj: Bio-protocol [Bio Protoc] 2021 Nov 20; Vol. 11 (22), pp. e4234. Date of Electronic Publication: 2021 Nov 20 (Print Publication: 2021).
DOI: 10.21769/BioProtoc.4234
Abstrakt: Visual impairments, notably loss of contrast sensitivity and color vision, were documented in Alzheimer's disease (AD) patients yet are critically understudied. This protocol describes a novel visual-stimuli four-arm maze (ViS4M; also called visual x-maze), which is a versatile x-shaped maze equipped with spectrum- and intensity-controlled light-emitting diode (LED) sources and dynamic grayscale objects. The ViS4M is designed to allow the assessment of color and contrast vision along with locomotor and cognitive functions in mice. In the color testing mode, the spectral distributions of the LED lights create four homogenous spaces that differ in chromaticity and luminance, corresponding to the mouse visual system. In the contrast sensitivity test, the four grayscale objects are placed in the middle of each arm, contrasting against the black walls and the white floors of the maze. Upon entering the maze, healthy wild-type (WT) mice tend to spontaneously alternate between arms, even under equiluminant conditions of illumination, suggesting that cognitively and visually intact mice use both color and brightness as cues to navigate the maze. Evaluation of the double-transgenic APP SWE /PS1 ΔE9 mouse model of AD (AD + mice) reveals substantial deficits to alternate in both color and contrast modes at an early age, when hippocampal-based memory and learning is still intact. Profiling of timespan, entries, and transition patterns between the different arms uncovers variable aging and AD-associated impairments in color discrimination and contrast sensitivity. The analysis of arm sequences of alternation reveals different pathways of exploration in young WT, old WT, and AD + mice, which can be used as color and contrast imprints of functionally intact versus impaired mice. Overall, we describe the utility of a novel visual x-maze test to identify behavioral changes in mice related to cognition, as well as color and contrast vision, with high precision and reproducibility. Graphic abstract: Exploratory behavior of AD + mice versus age- and sex-matched WT mice is tracked (top left: trajectory from a 5-min video file) in a novel visual-stimuli four-arm maze (ViS4M; also named visual x-maze) equipped with spectrum- and intensity-controlled LED sources or grayscale objects. Consecutive arm entries reveal that APP SWE /PS1 ΔE9 (AD + ) mice alternate less between arms, as opposed to WT mice. Sequence analysis, according to the three alternation pathways (depicted by white, yellow, and brown arrows) under different conditions of illumination, uncovers specific deficits linked to color vision in AD + mice, evidenced by a color imprint chart.
Competing Interests: Competing interestsMKH, YK and KLB are co-founders and stockholders of NeuroVision Imaging, Inc., 1395 Garden Highway, Suite 250, Sacramento, CA 95833, USA. MKH, YK and KLB are co-inventors on a patent "Visual stimuli maze test for detecting visual abnormalities in prodromal alzheimer's disease and in alzheimer's disease” application number US16/301,585, filing date 6/2/2017, licensed to Maze Engineers, 5250 Old Orchard Rd., Skokie, Illinois. Ariel Angel, Aharon Levy and Itschak Lamensdorf are former or current employees of Pharmaseed Ltd., Ness Ziona 74047, Israel. Jean-Philippe Vit and Dieu-Trang Fuchs have no competing interests.
(Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.)
Databáze: MEDLINE
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