Identification of microbes in wounds using near-infrared spectroscopy.

Autor: Yin M; Department of Burn and Plastic Surgery, Institute Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China., Li J; Department of Burn and Plastic Surgery, Institute Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China., Huang L; Institute of Fluid Physics, China Academy of Engineering Physics, Mianyang, Sichuan, China., Li Y; School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, China., Yuan M; Department of Burn Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China., Luo Y; Institute of Fluid Physics, China Academy of Engineering Physics, Mianyang, Sichuan, China., Armato U; Department of Burn and Plastic Surgery, Institute Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China; Human Histology and Embryology Unit, University of Verona Medical School, Strada Le Grazie 8, Verona, Italy., Zhang L; Department of Burn and Plastic Surgery, Institute Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China., Wei Y; Department of Burn and Plastic Surgery, Institute Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China., Li Y; Department of Burn and Plastic Surgery, Institute Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China., Deng J; Department of Burn and Plastic Surgery, Institute Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China., Wang P; School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, China. Electronic address: wangpin@cqu.edu.cn., Wu J; Department of Burn and Plastic Surgery, Institute Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China. Electronic address: junwupro@126.com.
Jazyk: angličtina
Zdroj: Burns : journal of the International Society for Burn Injuries [Burns] 2022 Jun; Vol. 48 (4), pp. 791-798. Date of Electronic Publication: 2021 Sep 17.
DOI: 10.1016/j.burns.2021.09.002
Abstrakt: Background: Rapid diagnosis of microbes in the burn wound is a big challenge in the medical field. Traditional biochemical detection techniques take hours or days to identify the species of contaminating and drug-resistant microbes. Near-infrared spectroscopy (NIRS) is evaluated to address the need for a fast and sensitive method for the detection of bacterial contamination in liquids.
Methods: Herin, we developed a novel technique which by using NIRS together with supporting vector machine (SVM), to identify the microbial species and drug-resistant microbes in LB medium, and to diagnose the wound colonization and wound infection models of pigs.
Results: The device could recognize 100% of seven kinds of microbes and 99.47% of the multi-drug resistant Staphylococcus aureus (S. aureus), with a concentration of 10 9 cfu ml -1 in LB medium. The accuracy of the microbial identification in colonized and infected wounds in-situ was 100%. The detection limit of NIRS with SVM for the detection of S. aureus and Escherichia coli (E. coli) was 10 1 cfu ml -1 in LB medium. Identification time was less than 5 s.
Conclusion: Our findings validate for the first time a novel technique aimed at the rapid, noncontacted, highly sensitive, and specific recognition of several microbial species including drug-resistant ones. This technique could represent a promising approach to identify diverse microbial species and a potential bedside device to rapidly diagnose infected wounds.
(Copyright © 2021 Elsevier Ltd and ISBI. All rights reserved.)
Databáze: MEDLINE
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