| Autor: |
Fahmy MA; Medical Parasitology Department, Theodor Bilharz Research Institute - TBRI, Giza, Egypt., Abdelaal AA; Medical Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.; Medical Parasitology Department, Faculty of Medicine, Armed Forces College of Medicine - AFCM, Cairo, Egypt., Hassan SI; Medical Parasitology Department, Theodor Bilharz Research Institute - TBRI, Giza, Egypt., Shalaby MA; Medical Parasitology Department, Theodor Bilharz Research Institute - TBRI, Giza, Egypt., Ismail MAM; Medical Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt., Khairy RA; Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt., Badawi MA; Pathology Department, Faculty of Medicine, National Research Institute, Giza, Egypt., Afife AA; College of Life Sciences, Faculty of Medicine, Leicester University, United Kingdom., Fadl HO; Medical Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. |
| Abstrakt: |
The present work aims to investigate the antiparasitic and the immunomodulating effects of nitazoxanide (NTZ) and ivermectin (IVC) alone or combined together or combined with selenium (Se), on Cryptosporidium infection in diabetic mice. The results revealed that the combined NTZ and IVC therapy achieved the highest reduction of fecal oocysts (92%), whereas single NTZ showed the lowest reduction (63%). Also, adding Se to either NTZ or IVC resulted in elevation of oocyst reduction from 63% to 71% and from 82% to 84% respectively. All treatment regimens, with the exception of NTZ monotherapy, showed a significant improvement in the intestinal histopathology, the highest score was in combined NTZ and IVC therapy. The unique results of immunohistochemistry in this study showed reversal of the normal CD4/CD8 T cell ratio in the infected untreated mice, however, following therapy it reverts back to a normal balanced ratio. The combined (NTZ+ IVC) treatment demonstrated the highest level of CD4 T cell expression. Taken together, NTZ and IVC combined therapy showed remarkable anti-parasitic and immunostimulatory effects, specifically towards the CD4 population that seem to be promising in controlling cryptosporidiosis in diabetic individuals. Further research is required to explore other effective treatment strategies for those comorbid patients. |
