Robust Agrobacterium -Mediated Transient Expression in Two Duckweed Species (Lemnaceae) Directed by Non-replicating, Replicating, and Cell-to-Cell Spreading Vectors.

Autor:	Peterson A; Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Collaborative Innovation Centre of Regional Modern Agriculture and Environmental Protection, School of Life Sciences, Huaiyin Normal University, Huai'an, China.; Institute of Cell Biology and Genetic Engineering, National Academy of Science of Ukraine, Kyiv, Ukraine., Kishchenko O; Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Collaborative Innovation Centre of Regional Modern Agriculture and Environmental Protection, School of Life Sciences, Huaiyin Normal University, Huai'an, China.; Institute of Cell Biology and Genetic Engineering, National Academy of Science of Ukraine, Kyiv, Ukraine., Zhou Y; Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Collaborative Innovation Centre of Regional Modern Agriculture and Environmental Protection, School of Life Sciences, Huaiyin Normal University, Huai'an, China., Vasylenko M; Institute of Cell Biology and Genetic Engineering, National Academy of Science of Ukraine, Kyiv, Ukraine., Giritch A; Nomad Bioscience GmbH, Halle, Germany., Sun J; Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Xuzhou, China., Borisjuk N; Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Collaborative Innovation Centre of Regional Modern Agriculture and Environmental Protection, School of Life Sciences, Huaiyin Normal University, Huai'an, China., Kuchuk M; Institute of Cell Biology and Genetic Engineering, National Academy of Science of Ukraine, Kyiv, Ukraine.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2021 Nov 04; Vol. 9, pp. 5. Date of Electronic Publication: 2021 Nov 04 (Print Publication: 2021).
DOI:	10.3389/fbioe.2021.761073
Abstrakt:	Plant-based transient expression systems have recognized potential for use as rapid and cost-effective alternatives to expression systems based on bacteria, yeast, insect, or mammalian cells. The free-floating aquatic plants of the Lemnaceae family (duckweed) have compact architecture and can be vegetatively propagated on low-cost nutrient solutions in aseptic conditions. These features provide an economically feasible opportunity for duckweed-based production of high-value products via transient expression of recombinant products in fully contained, controlled, aseptic and bio-safe conditions in accordance with the requirements for pharmaceutical manufacturing and environmental biosafety. Here, we demonstrated Agrobacterium -mediated high-yield transient expression of a reporter green fluorescent protein using deconstructed vectors based on potato virus X and sweet potato leaf curl virus, as well as conventional binary vectors, in two representatives of the Lemnaceae ( Spirodela polyrhiza and Landoltia punctata ). Aseptically cultivated duckweed populations yielded reporter protein accumulation of >1 mg/g fresh biomass, when the protein was expressed from a deconstructed potato virus X-based vector, which is capable of replication and cell-to-cell movement of the replicons in duckweed. The expression efficiency demonstrated here places duckweed among the most efficient host organisms for plant-based transient expression systems, with the additional benefits of easy scale-up and full containment. Competing Interests: AG is employed by Nomad Bioscience GmbH. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Peterson, Kishchenko, Zhou, Vasylenko, Giritch, Sun, Borisjuk and Kuchuk.)
Databáze:	MEDLINE
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