Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation.

Autor: Araújo TT; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil., Carvalho TS; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil., Dionizio A; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil., Debortolli ALB; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil., Ventura TMO; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil., Souza BM; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil., Feitosa CMVB; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil., Barbosa HAP; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil., Ribeiro CFDS; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil., Martini T; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil., Taira E; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil., Shibao PYT; Department of Genetics and Evolution, São Carlos Federal University, São Carlos, Brazil., Henrique-Silva F; Department of Genetics and Evolution, São Carlos Federal University, São Carlos, Brazil., Marchetto R; Department of Biochemistry and Technology, Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil., Buzalaf MAR; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil. Electronic address: mbuzalaf@fob.usp.br.
Jazyk: angličtina
Zdroj: Journal of dentistry [J Dent] 2022 Jan; Vol. 116, pp. 103874. Date of Electronic Publication: 2021 Nov 16.
DOI: 10.1016/j.jdent.2021.103874
Abstrakt: Objective: To study the proteomic alterations in the initial AEP after rinsing with CaneCPI-5, StN15 or Hb or their combination.
Materials and Methods: In five crossover phases, after prophylaxis, 10 volunteers in 5 consecutive days, rinsed (10 mL, 1 min) with the following solutions: deionized water (H 2 O- negative control- 1), 0.1 mg/mL CaneCPI-5 (2), 1.88×10 - 5 M StN15 (3), 1.0 mg/mL Hb (4) or their combination (5). The AEP formed after 3 min was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics.
Results: Rinsing with the proteins/peptide increased the amounts of proteins in the AEP. The total numbers of proteins identified after rinsing with CaneCPI-5, StN15, Hb or their combination versus water, were 131, 167, 148 and 142, respectively. The treatment with the proteins/peptide or their combination increased proteins that bind calcium, phosphate and interact with distinct proteins, as well as proteins with antimicrobial and acid-resistant properties, such as, Cornifin-B (7.7, 12.6, and 4.3-fold for CaneCPI-5, StN15 and Hb, respectively), isoforms of Cystatin (2.2-2.4-fold for CaneCPI-5 and StN15), Proline-rich-protein 4 (4.3-fold; StN15), Histatin-1 (2.8-fold; StN15) and Hemoglobin (7.7-25-fold for Hb and Combination). Immunoglobulin, Keratin and Histone were exclusively identified upon treatment with the proteins/peptide, alone or combined.
Conclusion: Rinsing with proteins/peptide, alone or combined, increased protective proteins in the initial AEP.
Clinical Relevance: Our results suggest that rinsing with the proteins/peptide or their combination increases the proteins capable of enhancing the protective function of the basal layer of AEP.
(Copyright © 2021. Published by Elsevier Ltd.)
Databáze: MEDLINE
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