Validation of Fenoterol to Study β2-Adrenoceptor Function in the Rat Urinary Bladder.
| Autor: | Erdogan BR; Department of Pharmacology, Faculty of Pharmacy, Ankara University, Ankara, Turkey.; Department of Pharmacology, Faculty of Pharmacy, Izmir Katip Celebi University, Izmir, Turkey., Yesilyurt ZE; Department of Pharmacology, Faculty of Pharmacy, Ankara University, Ankara, Turkey., Arioglu-Inan E; Department of Pharmacology, Faculty of Pharmacy, Ankara University, Ankara, Turkey., Michel MC; Department of Pharmacology, Johannes Gutenberg University, Mainz, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | Pharmacology [Pharmacology] 2022; Vol. 107 (1-2), pp. 116-121. Date of Electronic Publication: 2021 Nov 15. |
| DOI: | 10.1159/000519720 |
| Abstrakt: | Fenoterol is a β2-adrenoceptor (AR)-selective agonist that is commonly used to investigate relaxation responses mediated by β2-AR in smooth muscle preparations. Some data have questioned this because fenoterol had low potency in the rat urinary bladder when a muscarinic agonist was used as a pre-contraction agent and because some investigators proposed that fenoterol may act in part via β3-AR. We designed the present study to investigate whether fenoterol is a proper pharmacological tool to study β2-AR-mediated relaxation responses in the rat urinary bladder. Firstly, we have compared the effect of pre-contraction agents on fenoterol potency and found that fenoterol potency was about 1.5 log units greater against KCl than carbachol (pEC50 7.19 ± 0.66 and 5.62 ± 1.09 of KCl and of carbachol, respectively). To test the selectivity of fenoterol, we have determined the effects of the β2-AR antagonist ICI 118,551 and the β3-AR antagonist L 748,337 on relaxation responses to fenoterol. While 300 nM L 748,337 had little effect on the potency of fenoterol (pEC50 6.56 ± 0.25 and 6.33 ± 0.61 in the absence and presence of L 748,337, respectively), the relaxation curve for fenoterol was right-shifted in the presence 300 nM ICI 118,551 (pEC50 5.03 ± 0.18). Thus, we conclude that fenoterol is a proper pharmacological tool to assess β2-AR-mediated responses in the rat urinary bladder and most likely in other smooth-muscle preparations containing multiple subtypes of the β-AR. (© 2021 S. Karger AG, Basel.) |
| Databáze: | MEDLINE |
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