A New Tool for Analyses of Whole Genome Sequences Reveals Dissemination of Specific Strains of Vancomycin-Resistant Enterococcus faecium in a Hospital.

Autor:	Marbjerg L; Department of Clinical Research, University of Southern Denmark, Odense, Denmark., Stougaard CL; Department of Clinical Research, University of Southern Denmark, Odense, Denmark., Sørensen SG; Department of Clinical Research, University of Southern Denmark, Odense, Denmark., Thomsen AV; Department of Clinical Research, University of Southern Denmark, Odense, Denmark., Wang L; Department of Clinical Research, University of Southern Denmark, Odense, Denmark., Andersen L; Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark., Andersen TE; Department of Clinical Research, University of Southern Denmark, Odense, Denmark.; Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark., Kallipolitis B; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark., Kemp M; Department of Regional Health Research, University of Southern Denmark, Odense, Denmark.; Regional Department of Clinical Microbiology, Zealand University Hospital, Slagelse, Denmark.
Jazyk:	angličtina
Zdroj:	Frontiers in medicine [Front Med (Lausanne)] 2021 Oct 27; Vol. 8, pp. 733676. Date of Electronic Publication: 2021 Oct 27 (Print Publication: 2021).
DOI:	10.3389/fmed.2021.733676
Abstrakt:	A new easy-to-use online bioinformatic tool analyzing whole genome sequences of healthcare associated bacteria was used by a local infection control unit to retrospectively map genetic relationship of isolates of E. faecium carrying resistance genes to vancomycin in a hospital. Three clusters of isolates were detected over a period of 5 years, suggesting transmission between patients. Individual relatedness between isolates within each cluster was established by SNP analyses provided by the system. Genetic antimicrobial resistance mechanisms to antibiotics other than vancomycin were identified. The results suggest that the system is suited for hospital surveillance of E. faecium carrying resistance genes to vancomycin in settings with access to next Generation Sequencing without bioinformatic expertise for interpretation of the genome sequences. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Marbjerg, Stougaard, Sørensen, Thomsen, Wang, Andersen, Andersen, Kallipolitis and Kemp.)
Databáze:	MEDLINE
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