Survival of the nematophagous fungus Duddingtonia flagrans to in vitro segments of sheep gastrointestinal tract.

Autor: Céspedes-Gutiérrez E; Corporación Colombiana de Investigación Agropecuaria, AGROSAVIA, Sede Central, Mosquera, Colombia. Electronic address: ecespedes@agrosavia.co., Aragón DM; Universidad Nacional de Colombia, Sede Bogotá, Colombia. Electronic address: dmaragonn@unal.edu.co., Gómez-Álvarez MI; Corporación Colombiana de Investigación Agropecuaria, AGROSAVIA, Sede Central, Mosquera, Colombia. Electronic address: mgomeza@agrosavia.co., Cubides-Cárdenas JA; Corporación Colombiana de Investigación Agropecuaria, AGROSAVIA, Sede Tibaitatá, Mosquera, Colombia. Electronic address: jcubides@agrosavia.co., Cortés-Rojas DF; Corporación Colombiana de Investigación Agropecuaria, AGROSAVIA, Sede Central, Mosquera, Colombia. Electronic address: dfcortes@agrosavia.co.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2021 Dec; Vol. 231, pp. 108172. Date of Electronic Publication: 2021 Nov 10.
DOI: 10.1016/j.exppara.2021.108172
Abstrakt: The nematophagous fungus Duddingtonia flagrans is used in integrated management of gastrointestinal nematodes in ruminants. The chlamydospores of the fungus, orally administered, pass through the segments of the ruminant digestive tract and, in the feces, capture the nematodes preventing their migration to grasslands. The drastic conditions of the gastrointestinal segments can negatively affect the fungus' biocontrol activity. The aim of this study was to assess the effect of in vitro conditions of the sheep's main gastrointestinal segments on the concentration, viability and nematode predatory ability of D. flagrans chlamydospores. The segments evaluated separately in vitro were the oral cavity, rumen, abomasum, and small intestine. The results showed that chlamydospores concentration was not affected by exposure to the different segments. The viability of the chlamydospores after exposure to the oral cavity (2.53 × 10 6  CFU/mL) and small intestine (1.24 × 10 5  CFU/mL) was significantly lower than its control treatment, with values of 6.67 × 10 6  CFU/mL and 2.31 × 10 5  CFU/mL respectively. Nematode predatory ability after rumen exposure was reduced by 7% compared to the control treatment, by 25% after abomasum exposure and by 17% after small intestine. This study revealed the individual in vitro effect of each segment of ovine gastrointestinal tract on the integrity of this strain of the fungus D. flagrans affecting its viability and nematode predatory ability under the evaluated conditions. Delivery systems could be designed to protect chlamydospores considering the impact of each gastrointestinal segment.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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