Challenges and Methods for the Study of CPP Translocation Mechanisms.

Autor:	Walrant A; Sorbonne Université, École normale supérieure, PSL University, CNRS, Laboratoire des biomolécules, LBM, Paris, France., Illien F; Sorbonne Université, École normale supérieure, PSL University, CNRS, Laboratoire des biomolécules, LBM, Paris, France., Sagan S; Sorbonne Université, École normale supérieure, PSL University, CNRS, Laboratoire des biomolécules, LBM, Paris, France. sandrine.sagan@sorbonne-universite.fr., Rodriguez N; Sorbonne Université, École normale supérieure, PSL University, CNRS, Laboratoire des biomolécules, LBM, Paris, France.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2383, pp. 143-152.
DOI:	10.1007/978-1-0716-1752-6_9
Abstrakt:	Fluorescence-based methods are widely used to detect crossing of peptides across model or biological membranes. For membrane-active peptides, i.e., peptides that have strong membrane tropism, fluorescence experiments must be accompanied by relevant controls, otherwise they can lead to inconsistent interpretation and underestimation of their limitations. Here we describe how to prepare samples to study fluorescent peptide crossing droplet interface bilayer (model membrane) or cell membrane (biological membrane) and the pitfalls that can affect observational qualitative and quantitative data. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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