An siRNA-guided ARGONAUTE protein directs RNA polymerase V to initiate DNA methylation.

Autor: Sigman MJ; Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.; Donald Danforth Plant Science Center, St. Louis, MO, USA., Panda K; Donald Danforth Plant Science Center, St. Louis, MO, USA., Kirchner R; Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.; Medical Scientist Training Program, University of Wisconsin, Madison, WI, USA., McLain LL; Donald Danforth Plant Science Center, St. Louis, MO, USA., Payne H; Donald Danforth Plant Science Center, St. Louis, MO, USA.; Graduate Program in the School of Plant Sciences, University of Arizona, Tucson, AZ, USA., Peasari JR; Donald Danforth Plant Science Center, St. Louis, MO, USA.; Bioinformatics and Computational Biology Program, Saint Louis University, St. Louis, MO, USA., Husbands AY; Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA., Slotkin RK; Donald Danforth Plant Science Center, St. Louis, MO, USA. kslotkin@danforthcenter.org.; Division of Biological Sciences, University of Missouri, Columbia, MO, USA. kslotkin@danforthcenter.org., McCue AD; Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.; Donald Danforth Plant Science Center, St. Louis, MO, USA.
Jazyk: angličtina
Zdroj: Nature plants [Nat Plants] 2021 Nov; Vol. 7 (11), pp. 1461-1474. Date of Electronic Publication: 2021 Nov 08.
DOI: 10.1038/s41477-021-01008-7
Abstrakt: In mammals and plants, cytosine DNA methylation is essential for the epigenetic repression of transposable elements and foreign DNA. In plants, DNA methylation is guided by small interfering RNAs (siRNAs) in a self-reinforcing cycle termed RNA-directed DNA methylation (RdDM). RdDM requires the specialized RNA polymerase V (Pol V), and the key unanswered question is how Pol V is first recruited to new target sites without pre-existing DNA methylation. We find that Pol V follows and is dependent on the recruitment of an AGO4-clade ARGONAUTE protein, and any siRNA can guide the ARGONAUTE protein to the new target locus independent of pre-existing DNA methylation. These findings reject long-standing models of RdDM initiation and instead demonstrate that siRNA-guided ARGONAUTE targeting is necessary, sufficient and first to target Pol V recruitment and trigger the cycle of RdDM at a transcribed target locus, thereby establishing epigenetic silencing.
(© 2021. The Author(s).)
Databáze: MEDLINE
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