Potential of Human Neural Precursor Cells in Diabetic Retinopathy Therapeutics - Preclinical Model.

Autor: Saçaki CS; Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Research Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculties, Curitiba, Brazil., Mogharbel BF; Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Research Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculties, Curitiba, Brazil., Stricker PEF; Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Research Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculties, Curitiba, Brazil., Dziedzic DSM; Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Research Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculties, Curitiba, Brazil., Irioda AC; Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Research Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculties, Curitiba, Brazil., Perussolo MC; Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Research Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculties, Curitiba, Brazil., Somma AT; Veterinary Medicine Department, Federal University of Paraná, Curitiba, Brazil., Montiani-Ferreira F; Veterinary Medicine Department, Federal University of Paraná, Curitiba, Brazil., Moreno JCD; Veterinary Medicine Department, Federal University of Paraná, Curitiba, Brazil., Dornbusch P; Veterinary Medicine Department, Federal University of Paraná, Curitiba, Brazil., Sato M; Ophthalmology Department, Federal University of Paraná, Curitiba, Brazil., Shiokawa N; Ophthalmology Department, Federal University of Paraná, Curitiba, Brazil., de Noronha L; Pathology Department of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Curitiba, Brazil., Nagashima S; Pathology Department of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Curitiba, Brazil., Bacellar-Galdino M; Ophthy-DS, Kalamazoo, Michigan, USA., Franco CRC; Molecular Biology Department, Federal University of Paraná, Curitiba, Brazil., Abdelwahid E; Feinberg School of Medicine, Feinberg Cardiovascular Research Institute,Chicago, Illinois, USA., Athayde Teixeira de Carvalho K; Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Research Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculties, Curitiba, Brazil.
Jazyk: angličtina
Zdroj: Current eye research [Curr Eye Res] 2022 Mar; Vol. 47 (3), pp. 450-460. Date of Electronic Publication: 2021 Dec 06.
DOI: 10.1080/02713683.2021.2002909
Abstrakt: Purpose: This study aimed to evaluate a cell therapy strategy with human neural precursor cells (hNPCs) to treat diabetic retinopathy (DR) in Wistar rats induced to diabetes by injecting streptozotocin.
Material and Methods: The Wharton's jelly mesenchymal stem cells (WJ-MSCs) were isolated, expanded, and seeded onto a biopolymer substrate to develop neurospheres and obtain the hNPCs. The animals were divided into three groups: non-diabetic (ND) n = four, diabetic without treatment (DM) n = nine, and diabetic with cell therapy (DM + hNPCs) n = nine. After 8 weeks of diabetes induction and DR characteristics installed, intravitreal injection of hNPCs (1 × 10 6 cell/µL) was performed in the DM + hNPCs group. Optical Coherence Tomography (OCT) and Electroretinography (ERG) evaluations were conducted before and during diabetes and after cell therapy. Four weeks posttreatment, histopathological and immunohistochemistry analyses were performed.
Results: The repair of the retinal structures in the treated group (DM + hNPCs) was observed by increased thickness of neuroretinal layers, especially in the ganglion cell and photoreceptor layers, higher ERG oscillatory potentials (OPs) amplitudes, and transplanted hNPCs integration into the Retinal Pigment Epithelium.
Conclusions: The results indicate that hNPCs reduced DR progression by a neuroprotective effect and promoted retinal repair, making them potential candidates for regenerating the neuroretinal tissue.
Databáze: MEDLINE
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