Fluidics system for resolving concentration-dependent effects of dissolved gases on tissue metabolism.

Autor: Kamat V; University of Washington Medicine Diabetes Institute, University of Washington, Seattle, United States., Robbings BM; University of Washington Medicine Diabetes Institute, University of Washington, Seattle, United States.; Department of Biochemistry, University of Washington, Seattle, United States., Jung SR; University of Washington Medicine Diabetes Institute, University of Washington, Seattle, United States., Kelly J; VICI Metronics, Seattle, United States., Hurley JB; Department of Biochemistry, University of Washington, Seattle, United States., Bube KP; Department of Mathematics, University of Washington, Seattle, United States., Sweet IR; University of Washington Medicine Diabetes Institute, University of Washington, Seattle, United States.
Jazyk: angličtina
Zdroj: ELife [Elife] 2021 Nov 04; Vol. 10. Date of Electronic Publication: 2021 Nov 04.
DOI: 10.7554/eLife.66716
Abstrakt: Oxygen (O 2 ) and other dissolved gases such as the gasotransmitters H 2 S, CO, and NO affect cell metabolism and function. To evaluate effects of dissolved gases on processes in tissue, we developed a fluidics system that controls dissolved gases while simultaneously measuring parameters of electron transport, metabolism, and secretory function. We use pancreatic islets, retina, and liver from rodents to highlight its ability to assess effects of O 2 and H 2 S. Protocols aimed at emulating hypoxia-reperfusion conditions resolved a previously unrecognized transient spike in O 2 consumption rate (OCR) following replenishment of O 2 , and tissue-specific recovery of OCR following hypoxia. The system revealed both inhibitory and stimulatory effects of H 2 S on insulin secretion rate from isolated islets. The unique ability of this new system to quantify metabolic state and cell function in response to precise changes in dissolved gases provides a powerful platform for cell physiologists to study a wide range of disease states.
Competing Interests: VK, BR, SJ, JH, KB, IS No competing interests declared, JK through his employment at VICI Metronic, has a competing interest for the permeation tubes used in the study
(© 2021, Kamat et al.)
Databáze: MEDLINE
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