Antidiarrheal activity of Bridelia ferruginea bark methanolic extract involves modulation ATPases in mice and inhibition of muscarinic acetylcholine receptor (M3) and prostaglandin E2 receptor 3 (EP 3 ) in silico .

Autor: Omolaso BO; Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Nigeria., Adesanwo JK; Department of Chemistry, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria., Ishola AA; Central Research Laboratories Limited, Ilorin, Kwara State, Nigeria., Adegoke AG; Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Nigeria., Akingbule FO; Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Nigeria., Ipadeola YA; Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Nigeria., Adewole KE; Department of Biochemistry, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Ondo State, Nigeria.
Jazyk: angličtina
Zdroj: Journal of complementary & integrative medicine [J Complement Integr Med] 2021 Nov 03; Vol. 20 (4), pp. 757-771. Date of Electronic Publication: 2021 Nov 03 (Print Publication: 2023).
DOI: 10.1515/jcim-2021-0240
Abstrakt: Objectives: Diarrhea, an abnormal state in which the individual has about three or more daily bowel movements, is now considered one of the most challenging global public health problems. Using plant products, such as Bridelia ferruginea is an alternative treatment option. The objective of this study was to investigate the antidiarrheal activity of B. ferruginea bark methanolic extract (BfME) and the mechanisms involved.
Methods: BfME antidiarrheal activity was evaluated in mice model of castor oil-induced diarrhea and enteropooling. To evaluate motility, gastrointestinal transit time was carried out using phenol red meal, while intestinal activities of selected ATPases were also evaluated. Furthermore, the active components in BfME were detected by GC-MS analysis, while molecular docking of the most abundant compounds with muscarinic acetylcholine receptor (M3) and prostaglandin E2 receptor 3 (EP 3 ) were conducted.
Results: BfME at 400 and 800 mg/kg showed antidiarrheal activity by delaying onset of diarrhea, reduced gastrointestinal transit and increased intestinal activities of Na + K + -ATPase, Ca 2+ Mg 2+ -ATPase and Mg 2+ -ATPase. Molecular docking revealed that γ-sitosterol, α-amyrin, and stigmasterol have outstanding binding affinity for M3 and EP 3 .
Conclusions: In view of these results, the observed antidiarrheal activity possibly occurs via the activation of ATPases activities and inhibition of M3 and EP 3 .
(© 2021 Walter de Gruyter GmbH, Berlin/Boston.)
Databáze: MEDLINE
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