Prevalence, complete genome, and metabolic potentials of a phylogenetically novel cyanobacterial symbiont in the coral-killing sponge, Terpios hoshinota.

Autor:	Chen YH; Bioinformatics Program, Taiwan International Graduate Program, National Taiwan University, Taipei, Taiwan.; Bioinformatics Program, Institute of Information Science, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan.; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan., Chen HJ; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan., Yang CY; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan., Shiu JH; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan., Hoh DZ; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan.; Biodiversity Program, Taiwan International Graduate Program, Academia Sinica and National Taiwan Normal University, Taipei, Taiwan.; Department of Life Science, National Taiwan Normal University, Taipei, Taiwan., Chiang PW; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan., Chow WS; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan.; Biodiversity Program, Taiwan International Graduate Program, Academia Sinica and National Taiwan Normal University, Taipei, Taiwan.; Department of Life Science, National Taiwan Normal University, Taipei, Taiwan., Chen CA; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan.; Biodiversity Program, Taiwan International Graduate Program, Academia Sinica and National Taiwan Normal University, Taipei, Taiwan., Shih TH; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan., Lin SH; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan., Yang CM; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan., Reimer JD; Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, Japan.; Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa, Japan., Hirose E; Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, Japan., Iskandar BH; Department of Fishery Resources Utilization, Faculty of Fisheries and Marine Science, Bogor Agricultural University, Bogor, Indonesia., Huang H; Tropical Marine Biological Research Station in Hainan, Chinese Academy of Sciences, Sanya, China., Schupp PJ; Institute of Chemistry and Biology of the Marine Environment, University of Oldenburg, Wilhelmshaven, Germany., Tan CHJ; Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.; Institute of Oceanography and Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia., Yamashiro H; Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa, Japan., Liao MH; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan., Tang SL; Bioinformatics Program, Institute of Information Science, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan.; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan.; Biodiversity Program, Taiwan International Graduate Program, Academia Sinica and National Taiwan Normal University, Taipei, Taiwan.; Department of Life Science, National Taiwan Normal University, Taipei, Taiwan.
Jazyk:	angličtina
Zdroj:	Environmental microbiology [Environ Microbiol] 2022 Mar; Vol. 24 (3), pp. 1308-1325. Date of Electronic Publication: 2021 Oct 27.
DOI:	10.1111/1462-2920.15824
Abstrakt:	Terpios hoshinota is an aggressive, space-competing sponge that kills various stony corals. Outbreaks of this species have led to intense damage to coral reefs in many locations. Here, the first large-scale 16S rRNA gene survey across three oceans revealed that bacteria related to the taxa Prochloron, Endozoicomonas, SAR116, Ruegeria, and unclassified Proteobacteria were prevalent in T. hoshinota. A Prochloron-related bacterium was the most dominant and prevalent cyanobacterium in T. hoshinota. The complete genome of this uncultivated cyanobacterium and pigment analysis demonstrated that it has phycobiliproteins and lacks chlorophyll b, which is inconsistent with the definition of Prochloron. Furthermore, the cyanobacterium was phylogenetically distinct from Prochloron, strongly suggesting that it should be a sister taxon to Prochloron. Therefore, we proposed this symbiotic cyanobacterium as a novel species under the new genus Candidatus Paraprochloron terpiosi. Comparative genomic analyses revealed that 'Paraprochloron' and Prochloron exhibit distinct genomic features and DNA replication machinery. We also characterized the metabolic potentials of 'Paraprochloron terpiosi' in carbon and nitrogen cycling and propose a model for interactions between it and T. hoshinota. This study builds a foundation for the study of the T. hoshinota microbiome and paves the way for better understanding of ecosystems involving this coral-killing sponge. (© 2021 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu Plný text