Differential expression of transposable elements in the medaka melanoma model.

Autor: Helmprobst F; Physiological Chemistry, Biocenter, University of Würzburg, Würzburg, Germany.; Department of Neuropathology, Philipps-University Marburg, Marburg, Germany., Kneitz S; Physiological Chemistry, Biocenter, University of Würzburg, Würzburg, Germany.; Biochemistry and Cell Biology, Biocenter, University of Würzburg, Würzburg, Germany., Klotz B; Physiological Chemistry, Biocenter, University of Würzburg, Würzburg, Germany., Naville M; Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Université Lyon, Lyon, France., Dechaud C; Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Université Lyon, Lyon, France., Volff JN; Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Université Lyon, Lyon, France., Schartl M; The Xiphophorus Genetic Stock Center, Department of Chemistry and Biochemistry, Texas State University, San Marcos, Texas, United States of America.; Developmental Biochemistry, University of Würzburg, Würzburg, Germany.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2021 Oct 27; Vol. 16 (10), pp. e0251713. Date of Electronic Publication: 2021 Oct 27 (Print Publication: 2021).
DOI: 10.1371/journal.pone.0251713
Abstrakt: Malignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and the development of resistance against therapeutic approaches. Here we investigate as one source of genomic instability the contribution of activation of transposable elements (TEs) within the tumor. We used the well-established medaka melanoma model and RNA-sequencing to investigate the differential expression of TEs in wildtype and transgenic fish carrying melanoma. We constructed a medaka-specific TE sequence library and identified TE sequences that were specifically upregulated in tumors. Validation by qRT- PCR confirmed a specific upregulation of a LINE and an LTR element in malignant melanomas of transgenic fish.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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