| Autor: |
Connelly AR; Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA., Jeong BM; Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA., Coden ME; Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA., Cao JY; Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA., Chirkova T; Department of Pediatrics, Emory University School of Medicine and Children's Healthcare of Atlanta, Atlanta, GA 30322, USA., Rosas-Salazar C; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Cephus JY; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Anderson LJ; Department of Pediatrics, Emory University School of Medicine and Children's Healthcare of Atlanta, Atlanta, GA 30322, USA., Newcomb DC; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.; Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN 37203, USA., Hartert TV; Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN 37203, USA.; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37203, USA., Berdnikovs S; Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA. |
| Abstrakt: |
Respiratory syncytial virus (RSV) is a seasonal mucosal pathogen that infects the ciliated respiratory epithelium and results in the most severe morbidity in the first six months of life. RSV is a common cause of acute respiratory infection during infancy and is an important early-life risk factor strongly associated with asthma development. While this association has been repeatedly demonstrated, limited progress has been made on the mechanistic understanding in humans of the contribution of infant RSV infection to airway epithelial dysfunction. An active infection of epithelial cells with RSV in vitro results in heightened central metabolism and overall hypermetabolic state; however, little is known about whether natural infection with RSV in vivo results in lasting metabolic reprogramming of the airway epithelium in infancy. To address this gap, we performed functional metabolomics, 13C glucose metabolic flux analysis, and RNA-seq gene expression analysis of nasal airway epithelial cells (NAECs) sampled from infants between 2-3 years of age, with RSV infection or not during the first year of life. We found that RSV infection in infancy was associated with lasting epithelial metabolic reprogramming, which was characterized by (1) significant increase in glucose uptake and differential utilization of glucose by epithelium; (2) altered preferences for metabolism of several carbon and energy sources; and (3) significant sexual dimorphism in metabolic parameters, with RSV-induced metabolic changes most pronounced in male epithelium. In summary, our study supports the proposed phenomenon of metabolic reprogramming of epithelial cells associated with RSV infection in infancy and opens exciting new venues for pursuing mechanisms of RSV-induced epithelial barrier dysfunction in early life. |
