Effect of curcumin-encapsulated Pluronic ® F-127 over duo-species biofilm of Streptococcus mutans and Candida albicans.

Autor: Dos Santos DDL; Department of Dental Materials and Prosthodontics, School of Dentistry, Araraquara, São Paulo State University - UNESP, Araraquara, São Paulo, 14801-903, Brazil., Besegato JF; Department of Restorative Dentistry, School of Dentistry, Araraquara, São Paulo State University - UNESP, 1680 Humaitá St., MailBox: 331, Araraquara, São Paulo, 14.801-903, Brazil., de Melo PBG; Department of Restorative Dentistry, School of Dentistry, Araraquara, São Paulo State University - UNESP, 1680 Humaitá St., MailBox: 331, Araraquara, São Paulo, 14.801-903, Brazil., Junior JAO; Department of Drugs and Medicines, School of Pharmaceutical Sciences, Araraquara, São Paulo State University - UNESP, Araraquara, São Paulo, 14800-903, Brazil.; Graduate Program in Pharceutical Sciences, State University of Paraíba-UEPB, Paraíba, 58429-500, Brazil., Chorilli M; Department of Drugs and Medicines, School of Pharmaceutical Sciences, Araraquara, São Paulo State University - UNESP, Araraquara, São Paulo, 14800-903, Brazil., Deng D; Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam - ACTA, University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, Netherlands., Bagnato VS; Department of Physics and Materials Science, Physics Institute of São Carlos - IFSC, University of São Paulo - USP, São Carlos, São Paulo, 13566-590, Brazil., de Souza Rastelli AN; Department of Restorative Dentistry, School of Dentistry, Araraquara, São Paulo State University - UNESP, 1680 Humaitá St., MailBox: 331, Araraquara, São Paulo, 14.801-903, Brazil. alessandra.nara-souza-rastelli@unesp.br.
Jazyk: angličtina
Zdroj: Lasers in medical science [Lasers Med Sci] 2022 Apr; Vol. 37 (3), pp. 1775-1786. Date of Electronic Publication: 2021 Oct 19.
DOI: 10.1007/s10103-021-03432-9
Abstrakt: To assess the effect of curcumin-encapsulated Pluronic ® F-127 (Cur-Plu) during antimicrobial photodynamic therapy (aPDT) over duo-species biofilm of Streptococcus mutans and Candida albicans. Thermal analysis, optical absorption, and fluorescence spectroscopy were evaluated. Minimum inhibitory concentration (MIC) and minimum bactericidal/fungal concentration were obtained. The biofilms were cultured for 48 h at 37 °C and treated according to the groups: P + M + L + (photosensitizer encapsulated with Pluronic ® F-127 + light); P + D + L + (photosensitizer incorporated in 1% DMSO + light); P - M + L + (no Pluronic ® F-127 + light); P - D + L + (1% DMSO + light); P - L + (Milli-Q water + light); P + M + L - (photosensitizer encapsulated with Pluronic ® F-127 no light); P + D + L - (photosensitizer in 1% DMSO, no light); P - M + L - (Pluronic ® F-127 no light); P - D + L - (1% DMSO, no light); P - L - (Milli-Q water, no light; negative control group); CHX (0.2% chlorhexidine, positive control group); and NYS (Nystatin). Dark incubation of 5 min was used. The groups that received aPDT were irradiated by blue LED (460 nm, 15 J/cm 2 ). Cell viability of the biofilms was performed by colony-forming units (CFU/mL) and confocal microscopy. Two-way ANOVA followed by Tukey's post hoc test was used at a significance level of 5%. P + D + L + and P + M + L + groups exhibited better log-reduction for both Candida albicans and Streptococcus mutans biofilms than P - M + L + , P - L + , and P - D + L + experimental groups. Furthermore, P + M + L + and P + D + L + showed greater reduction for Candida albicans than for Streptococcus mutans. aPDT mediated by Cur-Plu can be a potential strategy for biofilm control against duo-species biofilm of Streptococcus mutans and Candida albicans.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)
Databáze: MEDLINE
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