Role of the major determinant of polar flagellation FlhG in the endoflagella-containing spirochete Leptospira.

Autor: Fule L; Institut Pasteur, Biology of Spirochetes Unit, Paris, France.; Pasteur International Unit, Integrative Microbiology of Zoonotic Agents, Institut Pasteur de Montevideo, Montevideo, Uruguay/Institut Pasteur, Paris, France.; Université de Paris, Paris, France., Halifa R; Institut Pasteur, Biology of Spirochetes Unit, Paris, France., Fontana C; Boehringer Ingelheim Santé Animale, Saint Priest, France., Sismeiro O; Transcriptome and Epigenome Platform, Biomics, Center for Technological Resources and Research (C2RT), Institut Pasteur, Paris, France., Legendre R; Transcriptome and Epigenome Platform, Biomics, Center for Technological Resources and Research (C2RT), Institut Pasteur, Paris, France.; Bioinformatics and Biostatistics Hub, Department of Computational Biology, Institut Pasteur, Paris, France., Varet H; Transcriptome and Epigenome Platform, Biomics, Center for Technological Resources and Research (C2RT), Institut Pasteur, Paris, France.; Bioinformatics and Biostatistics Hub, Department of Computational Biology, Institut Pasteur, Paris, France., Coppée JY; Transcriptome and Epigenome Platform, Biomics, Center for Technological Resources and Research (C2RT), Institut Pasteur, Paris, France., Murray GL; Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Victoria, Australia., Adler B; Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Victoria, Australia., Hendrixson DR; Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Buschiazzo A; Pasteur International Unit, Integrative Microbiology of Zoonotic Agents, Institut Pasteur de Montevideo, Montevideo, Uruguay/Institut Pasteur, Paris, France.; Laboratory of Molecular and Structural Microbiology, Institut Pasteur de Montevideo, Montevideo, Uruguay., Guo S; Microbial Sciences Institute & Department of Microbial Pathogenesis, Yale School of Medicine, New Haven, Connecticut, USA., Liu J; Microbial Sciences Institute & Department of Microbial Pathogenesis, Yale School of Medicine, New Haven, Connecticut, USA., Picardeau M; Institut Pasteur, Biology of Spirochetes Unit, Paris, France.; Pasteur International Unit, Integrative Microbiology of Zoonotic Agents, Institut Pasteur de Montevideo, Montevideo, Uruguay/Institut Pasteur, Paris, France.
Jazyk: angličtina
Zdroj: Molecular microbiology [Mol Microbiol] 2021 Nov; Vol. 116 (5), pp. 1392-1406. Date of Electronic Publication: 2021 Oct 30.
DOI: 10.1111/mmi.14831
Abstrakt: Spirochetes can be distinguished from other bacteria by their spiral-shaped morphology and subpolar periplasmic flagella. This study focused on FlhF and FlhG, which control the spatial and numerical regulation of flagella in many exoflagellated bacteria, in the spirochete Leptospira. In contrast to flhF which seems to be essential in Leptospira, we demonstrated that flhG - mutants in both the saprophyte L. biflexa and the pathogen L. interrogans were less motile than the wild-type strains in gel-like environments but not hyperflagellated as reported previously in other bacteria. Cryo-electron tomography revealed that the distance between the flagellar basal body and the tip of the cell decreased significantly in the flhG - mutant in comparison to wild-type and complemented strains. Additionally, comparative transcriptome analyses of L. biflexa flhG - and wild-type strains showed that FlhG acts as a negative regulator of transcription of some flagellar genes. We found that the L. interrogans flhG - mutant was attenuated for virulence in the hamster model. Cross-species complementation also showed that flhG is not interchangeable between species. Our results indicate that FlhF and FlhG in Leptospira contribute to governing cell motility but our data support the hypothesis that FlhF and FlhG function differently in each bacterial species, including among spirochetes.
(© 2021 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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