Finger sweat analysis enables short interval metabolic biomonitoring in humans.

Autor: Brunmair J; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria., Gotsmy M; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria., Niederstaetter L; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria., Neuditschko B; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria.; Department of Inorganic Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria., Bileck A; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria.; Joint Metabolome Facility, University and Medical University of Vienna, Vienna, Austria., Slany A; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria., Feuerstein ML; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria., Langbauer C; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria., Janker L; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria.; Joint Metabolome Facility, University and Medical University of Vienna, Vienna, Austria., Zanghellini J; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria., Meier-Menches SM; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria.; Department of Inorganic Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria.; Joint Metabolome Facility, University and Medical University of Vienna, Vienna, Austria., Gerner C; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria. christopher.gerner@univie.ac.at.; Joint Metabolome Facility, University and Medical University of Vienna, Vienna, Austria. christopher.gerner@univie.ac.at.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Oct 13; Vol. 12 (1), pp. 5993. Date of Electronic Publication: 2021 Oct 13.
DOI: 10.1038/s41467-021-26245-4
Abstrakt: Metabolic biomonitoring in humans is typically based on the sampling of blood, plasma or urine. Although established in the clinical routine, these sampling procedures are often associated with a variety of compliance issues, which are impeding time-course studies. Here, we show that the metabolic profiling of the minute amounts of sweat sampled from fingertips addresses this challenge. Sweat sampling from fingertips is non-invasive, robust and can be accomplished repeatedly by untrained personnel. The sweat matrix represents a rich source for metabolic phenotyping. We confirm the feasibility of short interval sampling of sweat from the fingertips in time-course studies involving the consumption of coffee or the ingestion of a caffeine capsule after a fasting interval, in which we successfully monitor all known caffeine metabolites as well as endogenous metabolic responses. Fluctuations in the rate of sweat production are accounted for by mathematical modelling to reveal individual rates of caffeine uptake, metabolism and clearance. To conclude, metabotyping using sweat from fingertips combined with mathematical network modelling shows promise for broad applications in precision medicine by enabling the assessment of dynamic metabolic patterns, which may overcome the limitations of purely compositional biomarkers.
(© 2021. The Author(s).)
Databáze: MEDLINE
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