Na + i /K + i imbalance contributes to gene expression in endothelial cells exposed to elevated NaCl.
| Autor: | Fedorov DA; Faculty of Biology, Lomonosov Moscow State University, Moscow, 1-12 Leninskie Gory, 119234, Russia., Sidorenko SV; Faculty of Biology, Lomonosov Moscow State University, Moscow, 1-12 Leninskie Gory, 119234, Russia., Yusipovich AI; Faculty of Biology, Lomonosov Moscow State University, Moscow, 1-12 Leninskie Gory, 119234, Russia., Parshina EY; Faculty of Biology, Lomonosov Moscow State University, Moscow, 1-12 Leninskie Gory, 119234, Russia., Tverskoi AM; Faculty of Biology, Lomonosov Moscow State University, Moscow, 1-12 Leninskie Gory, 119234, Russia.; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 32 Vavilova Street, 119991, Russia., Abramicheva PA; Faculty of Biology, Lomonosov Moscow State University, Moscow, 1-12 Leninskie Gory, 119234, Russia., Maksimov GV; Faculty of Biology, Lomonosov Moscow State University, Moscow, 1-12 Leninskie Gory, 119234, Russia., Orlov SN; Faculty of Biology, Lomonosov Moscow State University, Moscow, 1-12 Leninskie Gory, 119234, Russia., Lopina OD; Faculty of Biology, Lomonosov Moscow State University, Moscow, 1-12 Leninskie Gory, 119234, Russia., Klimanova EA; Faculty of Biology, Lomonosov Moscow State University, Moscow, 1-12 Leninskie Gory, 119234, Russia. |
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| Jazyk: | angličtina |
| Zdroj: | Heliyon [Heliyon] 2021 Sep 29; Vol. 7 (9), pp. e08088. Date of Electronic Publication: 2021 Sep 29 (Print Publication: 2021). |
| DOI: | 10.1016/j.heliyon.2021.e08088 |
| Abstrakt: | High-salt consumption contributes to the development of hypertension and is considered an independent risk factor for vascular remodelling, cardiac hypertrophy and stroke incidence. Alterations in NO production, inflammation and endothelial cell stiffening are considered now as plausible mediators of cardiovascular dysfunction. We studied early responses of endothelial cells (HUVEC) caused by a moderate increase in extracellular sodium concentration. Exposure of HUVEC to elevated sodium within the physiological range up to 24 h is accompanied by changes in monovalent cations fluxes and Na,K-ATPase activation, and, in turn, results in a significant decrease in the content of PTGS2 , IL6 and IL1LR1 mRNAs. The expression of NOS3 and FOS genes, as well as the abundance of cytosolic and nuclear NFAT5 protein, remained unchanged. We assessed the mechanical properties of endothelial cells by estimating Young's modulus and equivalent elastic constant using atomic force and interference microscopy, respectively. These parameters were unaffected by elevated-salt exposure for 24 h. The data obtained suggest that even small and short-term elevations of extracellular sodium concentration affect the expression of genes involved in the control of endothelial function through the Na + Competing Interests: The authors declare no conflict of interest. (© 2021 The Author(s).) |
| Databáze: | MEDLINE |
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