Impact of donor nutritional balance on the growth and development of mesenchymal stem cells from caprine umbilical cord Wharton´s jelly.

Autor: Alves JPM; School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, CE, 60.714-903, Brazil., Rossetto R; School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, CE, 60.714-903, Brazil., Fernandes CCL; School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, CE, 60.714-903, Brazil., Montenegro AR; School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, CE, 60.714-903, Brazil., Marques ITO; School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, CE, 60.714-903, Brazil., Cavalcanti CM; School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, CE, 60.714-903, Brazil., Bezerra AF; School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, CE, 60.714-903, Brazil., Rodrigues APR; School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, CE, 60.714-903, Brazil., Bertolini M; School of Veterinary Medicine, Federal University of Rio Grande Do Sul (UFRGS), Porto Alegre, RS, 90040-060, Brazil., Rondina D; School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, CE, 60.714-903, Brazil. davide.rondina@uece.br.
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2022 Feb; Vol. 46 (1), pp. 169-182. Date of Electronic Publication: 2021 Oct 09.
DOI: 10.1007/s11259-021-09843-x
Abstrakt: Mesenchymal stem cells (MSCs) from the umbilical cord (UC) have aroused considerable interest. However, little is known about the maternal effect on these cells. The aim of this study was to verify the impact of the nutritional status of donor goats on the growth and differentiation of MSCs from the UC. At parturition, 19 goats were grouped based on their low or high body mass index (low BMI, LBMI, n = 9; and high BMI, HBMI, n = 10). UCs were collected during delivery and Wharton's jelly (WJ) fragments cultured. WJ-MSCs were differentiated into osteocytes, adipocytes, chondrocytes, and the population doubling time (PDT) was determined. Samples of WJ-MSCs were also used to verify the expression of the CD90, CD73, CD34, CD45, and CD105 genes. Media used for WJ-MSC primary cultures were analyzed using near-infrared spectroscopy. The lag phase was 7.5 ± 0.6 days and the entire culture took 26.7 ± 1.3 days, with a cell proliferation rate of 8.500 cells/day. The mean PDT from subculture was 30.0 ± 0.7 h. The CD105 gene was sub-expressed in LBMI, and the spectra of the spent media from the second to fourth day of WJ-MSC primary culture were segregated into negative scores by multivariate analysis. We conclude that, in goats, the nutritional balance of the donor did not affect the in vitro growth of MSCs derived from the UC. However, the molecular profile observed in the low BMI group suggests that the use of MSCs for therapeutic purposes should be considered more carefully.
(© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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