Proteomic pathways to metabolic disease and type 2 diabetes in the pancreatic islet.

Autor:	Yau B; Discipline of Physiology, School of Medical Sciences, Charles Perkins Centre, University of Sydney, Camperdown 2006, Australia., Naghiloo S; Discipline of Physiology, School of Medical Sciences, Charles Perkins Centre, University of Sydney, Camperdown 2006, Australia., Diaz-Vegas A; School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, NSW, Australia., Carr AV; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA., Van Gerwen J; School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, NSW, Australia., Needham EJ; School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, NSW, Australia., Jevon D; Discipline of Physiology, School of Medical Sciences, Charles Perkins Centre, University of Sydney, Camperdown 2006, Australia., Chen SY; Department of Biotechnology and Biomolecular Sciences, University of New South Wales, Kensington, NSW 2052, Australia., Hoehn KL; Department of Biotechnology and Biomolecular Sciences, University of New South Wales, Kensington, NSW 2052, Australia.; Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA., Brandon AE; Discipline of Physiology, School of Medical Sciences, Charles Perkins Centre, University of Sydney, Camperdown 2006, Australia., Macia L; Discipline of Physiology, School of Medical Sciences, Charles Perkins Centre, University of Sydney, Camperdown 2006, Australia., Cooney GJ; Discipline of Physiology, School of Medical Sciences, Charles Perkins Centre, University of Sydney, Camperdown 2006, Australia., Shortreed MR; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA., Smith LM; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA., Keller MP; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA., Thorn P; Discipline of Physiology, School of Medical Sciences, Charles Perkins Centre, University of Sydney, Camperdown 2006, Australia., Larance M; School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, NSW, Australia., James DE; Discipline of Physiology, School of Medical Sciences, Charles Perkins Centre, University of Sydney, Camperdown 2006, Australia.; School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, NSW, Australia., Humphrey SJ; School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, NSW, Australia., Kebede MA; Discipline of Physiology, School of Medical Sciences, Charles Perkins Centre, University of Sydney, Camperdown 2006, Australia.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2021 Sep 09; Vol. 24 (10), pp. 103099. Date of Electronic Publication: 2021 Sep 09 (Print Publication: 2021).
DOI:	10.1016/j.isci.2021.103099
Abstrakt:	Pancreatic islets are essential for maintaining physiological blood glucose levels, and declining islet function is a hallmark of type 2 diabetes. We employ mass spectrometry-based proteomics to systematically analyze islets from 9 genetic or diet-induced mouse models representing a broad cross-section of metabolic health. Quantifying the islet proteome to a depth of >11,500 proteins, this study represents the most detailed analysis of mouse islet proteins to date. Our data highlight that the majority of islet proteins are expressed in all strains and diets, but more than half of the proteins vary in expression levels, principally due to genetics. Associating these varied protein expression levels on an individual animal basis with individual phenotypic measures reveals islet mitochondrial function as a major positive indicator of metabolic health regardless of strain. This compendium of strain-specific and dietary changes to mouse islet proteomes represents a comprehensive resource for basic and translational islet cell biology. Competing Interests: The authors declare no competing financial or non-financial interests. (© 2021 The Author(s).)
Databáze:	MEDLINE
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