Metagenomic evidence for co-occurrence of antibiotic, biocide and metal resistance genes in pigs.

Autor: Li X; Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark., Rensing C; Institute of Environmental Microbiology, College of Resources and Environment, Fujian Agriculture & Forestry University, Fuzhou 350002, China., Vestergaard G; Section of Bioinformatics, Department of Health Technology, Technical University of Denmark, 2800 Kgs, Lyngby, Denmark., Arumugam M; Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark., Nesme J; Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark., Gupta S; Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark., Brejnrod AD; Skaggs School of Pharmacy, University of California San Diego, La Jolla, United States. Electronic address: sjs@bio.ku.dk., Sørensen SJ; Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark.
Jazyk: angličtina
Zdroj: Environment international [Environ Int] 2022 Jan; Vol. 158, pp. 106899. Date of Electronic Publication: 2021 Sep 28.
DOI: 10.1016/j.envint.2021.106899
Abstrakt: Antibiotic-resistant pathogens constitute an escalating public health concern. Hence a better understanding of the underlying processes responsible for this expansion is urgently needed. Co-selection of heavy metal/biocide and antibiotic resistance genes (ARGs) has been suggested as one potential mechanism promoting the proliferation of antimicrobial resistance (AMR). This paper aims to elucidate this interplay and exploit differences in antibiotic usage to infer patterns of co-selection by the non-antibiotic factors metals and biocides in the context of pig farming. We examined 278 gut metagenomes from pigs with continuous antibiotic exposure, only at weaning and at no exposure. Metals as growth promoters and biocides as disinfectants are currently used with little restrictions in stock farming. The pigs under continuous antibiotic exposure displayed the highest co-occurrence of ARGs and other genetic elements while the pigs under limited use of antibiotics still showed abundant co-occurrences. Pathogens belonging to Enterobacteriaceae displayed increased co-occurrence phenomena, suggesting that this maintenance is not a random selection process from a mobilized pool but pertains to specific phylogenetic clades. These results suggest that metals and biocides displayed strong selective pressures on ARGs exerted by intensive farming, regardless of the current use of antibiotics.
(Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: