Bone marrow-derived mesenchymal stem cells combined with gonadotropin therapy restore postnatal oogenesis of chemo-ablated ovaries in rats via enhancing very small embryonic-like stem cells.

Autor: Ebrahim N; Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha, Egypt.; Stem Cell Unit, Faculty of Medicine, Benha University, Benha, Egypt., Al Saihati HA; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hafr Albatin, Al-Batin, Saudi Arabia., Shaman A; Obstetrics and Gynecology Medical College, Tabuk University, Tabuk, Saudi Arabia., Dessouky AA; Department of Medical Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt., Farid AS; Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt., Hussien NI; Department of Medical Physiology, Faculty of Medicine, Benha University, Benha, Egypt., Mostafa O; Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha, Egypt., Seleem Y; Department of Clinical Pharmacology, Faculty of Medicine, Benha University, Benha, Egypt., Sabry D; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Badr University, Cairo, Egypt., Saad AS; Department of Obstetrics & Gynecology, Faculty of Medicine, Benha University, Benha, Egypt., Emam HT; Department of Clinical Pharmacology, Faculty of Medicine, Benha University, Benha, Egypt., Hassouna A; School of Interprofessional Health Studies, Faculty of Health and Environmental Sciences, AUT University, Auckland, New Zealand., Badr OAM; Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Benha, Egypt., Saffaf BA; Department of pharmacology, Faculty of Pharmacy, Future University, Cairo, Egypt., Forsyth NR; Guy Hilton Research Laboratories, School of Pharmacy and Bioengineering, Faculty of Medicine and Health Sciences, Keele University, Keele, UK., Salim RF; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University, Benha, Qalyubia, 13512, Egypt. rabab.bio@yahoo.com.
Jazyk: angličtina
Zdroj: Stem cell research & therapy [Stem Cell Res Ther] 2021 Sep 27; Vol. 12 (1), pp. 517. Date of Electronic Publication: 2021 Sep 27.
DOI: 10.1186/s13287-021-02415-5
Abstrakt: Background: Very small embryonic-like stem cells (VSELs) are a rare population within the ovarian epithelial surface. They contribute to postnatal oogenesis as they have the ability to generate immature oocytes and resist the chemotherapy. These cells express markers of pluripotent embryonic and primordial germ cells.
Objective: We aimed to explore the capability of VSELs in restoring the postnatal oogenesis of chemo-ablated rat ovaries treated with bone marrow-derived mesenchymal stem cells (BM-MSCs) combined with pregnant mare serum gonadotropin (PMSG).
Methods: Female albino rats were randomly assigned across five groups: I (control), II (chemo-ablation), III (chemo-ablation + PMSG), IV (chemo-ablation + MSCs), and V (chemo-ablation + PMSG + MSCs). Postnatal oogenesis was assessed through measurement of OCT4, OCT4A, Scp3, Mvh, Nobox, Dazl4, Nanog, Sca-1, FSHr, STRA8, Bax, miR143, and miR376a transcript levels using qRT-PCR. Expression of selected key proteins were established as further confirmation of transcript expression changes. Histopathological examination and ovarian hormonal assessment were determined.
Results: Group V displayed significant upregulation of all measured genes when compared with group II, III or IV. Protein expression confirmed the changes in transcript levels as group V displayed the highest average density in all targeted proteins. These results were confirmed histologically by the presence of cuboidal germinal epithelium, numerous primordial, unilaminar, and mature Graafian follicles in group V.
Conclusion: VSELs can restore the postnatal oogenesis in chemo-ablated ovaries treated by BM-MSCs combined with PMSG.
(© 2021. The Author(s).)
Databáze: MEDLINE
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