Sand fly fauna and molecular detection of Leishmania species and blood meal sources in different rural environments in western Amazon.

Autor: Costa GDS; Programa de Pós-Graduação em Biologia Experimental, Fundação Universidade Federal de Rondônia, BR 364, Km 9.5, Porto Velho, RO 76801-059, Brazil; Laboratório de Entomologia, Fiocruz Rondônia, Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil. Electronic address: glaucilene.gsc@gmail.com., Júnior AMP; Laboratório de Entomologia, Fiocruz Rondônia, Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil; Instituto Nacional de Epidemiologia da Amazônia Ocidental (INCT-EpiAMO), Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil., Castro TS; Laboratório de Entomologia, Fiocruz Rondônia, Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil., de Paulo PFM; Laboratório de Entomologia, Fiocruz Rondônia, Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil., Ferreira GEM; Laboratório de EpiGenética, Fiocruz Rondônia, Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil., Medeiros JF; Laboratório de Entomologia, Fiocruz Rondônia, Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil; Instituto Nacional de Epidemiologia da Amazônia Ocidental (INCT-EpiAMO), Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2021 Dec; Vol. 224, pp. 106150. Date of Electronic Publication: 2021 Sep 22.
DOI: 10.1016/j.actatropica.2021.106150
Abstrakt: Cutaneous Leishmaniasis (CL) is a disease caused by Leishmania species and transmitted by the bites of infected female sand fly species. The diversity of these insects in Rondônia State (where CL is the predominant form) is large but unexplored, and consequently the vector species are unknown. The purpose of this study was to evaluate the sand fly fauna in two environments (forest fragment and peridomicile) in rural areas of four municipalities of the state, DNA amplification of Leishmania species and the presence of blood meal sources for these insects. After identifying the species, sample coverage was applied to estimate the fauna coverage in each environment. Females were used to amplify and detect Leishmania DNA and blood meal sources, then posteriorly identified by sequencing. A total of 1706 individuals were included in 61 species, which was a sample coverage of 97% for the forest fragments (56 species), whereas 98% was observed (32 species) in peridomiciles. Next, 41 pools were prepared from 1227 females, and none were positive for Leishmania DNA. We observed 160 engorged females (forest fragments: 21, peridomiciles: 139) belonging to females of the Antunesi complex and another eight species. Two of these females were positive for Leishmania braziliensis-DNA: one in the Antunesi complex and one in Psychodopygus hirsutus. Seven blood meal sources were identified by sequencing analysis: Bos taurus and Sus scrofa from the peridomiciles, and Dasypus novemcinctus, Pecari tajacu, Philander canus, Plecturocebus bernhardi, and Tamandua tetradactyla from the forest fragments. Our data confirmed the feeding behavior of field-caught sand flies and could contribute to our understanding about local vectors and possible reservoirs in the transmission of Leishmania spp.
(Copyright © 2021. Published by Elsevier B.V.)
Databáze: MEDLINE
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