First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity.

Autor: Gwiazdowska A; Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679, Warsaw, Poland., Karpińska O; Institute of Forest Sciences, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776, Warsaw, Poland., Kamionka-Kanclerska K; Institute of Forest Sciences, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776, Warsaw, Poland., Rowiński P; Institute of Forest Sciences, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776, Warsaw, Poland., Panagiotopoulou H; Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679, Warsaw, Poland., Pomorski JJ; Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679, Warsaw, Poland., Broughton RK; UK Centre for Ecology and Hydrology, Maclean Building, Benson Lane, Crowmarsh Gifford, Wallingford, OX10 8BB, UK., da Silva LFP; CBIO-InBIO Campus Agrário de Vairão Rua Padre Armando Quintas, nº7, 4485-661, Vila do Conde, Portugal., Rutkowski R; Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679, Warsaw, Poland. robertrut@miiz.waw.pl.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Sep 23; Vol. 11 (1), pp. 18962. Date of Electronic Publication: 2021 Sep 23.
DOI: 10.1038/s41598-021-98364-3
Abstrakt: The European Robin is a small passerine bird associated with woodlands of Eurasia and North Africa. Despite being relatively widespread and common, little is known of the species' breeding biology and genetic diversity. We used Next Generation Sequencing (NGS) to develop and characterize microsatellite markers for the European Robin, designing three multiplex panels to amplify 14 microsatellite loci. The level of polymorphism and its value for assessing parentage and genetic structure was estimated based on 119 individuals, including seven full families and 69 unrelated individuals form Poland's Białowieża Primaeval Forest and an additional location in Portugal. All markers appeared to be highly variable. Analysis at the family level confirmed a Mendelian manner of inheritance in the investigated loci. Genetic data also revealed evidence for extra-pair paternity in one family. The set of markers that we developed are proven to be valuable for analysis of the breeding biology and population genetics of the European Robin.
(© 2021. The Author(s).)
Databáze: MEDLINE
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