Tracing Transmission of Sin Nombre Virus and Discovery of Infection in Multiple Rodent Species.

Autor:	Goodfellow SM; Center for Global Health, Department of Internal Medicine, University of New Mexicogrid.266832.b Health Sciences Center, Albuquerque, New Mexico, USA., Nofchissey RA; Center for Global Health, Department of Internal Medicine, University of New Mexicogrid.266832.b Health Sciences Center, Albuquerque, New Mexico, USA., Schwalm KC; Department of Pediatrics, University of New Mexicogrid.266832.b Health Sciences Center, Albuquerque, New Mexico, USA., Cook JA; Museum of Southwestern Biology, Biology Department, University of New Mexicogrid.266832.b, Albuquerque, New Mexico, USA., Dunnum JL; Museum of Southwestern Biology, Biology Department, University of New Mexicogrid.266832.b, Albuquerque, New Mexico, USA., Guo Y; Comprehensive Cancer Center, Department of Internal Medicine, University of New Mexicogrid.266832.b Health Sciences Center, Albuquerque, New Mexico, USA., Ye C; Center for Global Health, Department of Internal Medicine, University of New Mexicogrid.266832.b Health Sciences Center, Albuquerque, New Mexico, USA., Mertz GJ; Center for Global Health, Department of Internal Medicine, University of New Mexicogrid.266832.b Health Sciences Center, Albuquerque, New Mexico, USA., Chandran K; Albert Einstein College of Medicine, Department of Microbiology and Immunology, Bronx, New York, USA., Harkins M; Division of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, University of New Mexicogrid.266832.b Health Sciences Center, Albuquerque, New Mexico, USA., Domman DB; Center for Global Health, Department of Internal Medicine, University of New Mexicogrid.266832.b Health Sciences Center, Albuquerque, New Mexico, USA., Dinwiddie DL; Department of Pediatrics, University of New Mexicogrid.266832.b Health Sciences Center, Albuquerque, New Mexico, USA., Bradfute SB; Center for Global Health, Department of Internal Medicine, University of New Mexicogrid.266832.b Health Sciences Center, Albuquerque, New Mexico, USA.
Jazyk:	angličtina
Zdroj:	Journal of virology [J Virol] 2021 Nov 09; Vol. 95 (23), pp. e0153421. Date of Electronic Publication: 2021 Sep 22.
DOI:	10.1128/JVI.01534-21
Abstrakt:	Sin Nombre orthohantavirus (SNV), a negative-sense, single-stranded RNA virus that is carried and transmitted by the North American deer mouse Peromyscus maniculatus, can cause infection in humans through inhalation of aerosolized excreta from infected rodents. This infection can lead to hantavirus cardiopulmonary syndrome (HCPS), which has an ∼36% case-fatality rate. We used reverse transcriptase quantitative PCR (RT-qPCR) to confirm SNV infection in a patient and identified SNV in lung tissues in wild-caught rodents from potential sites of exposure. Using viral whole-genome sequencing (WGS), we identified the likely site of transmission and discovered SNV in multiple rodent species not previously known to carry the virus. Here, we report, for the first time, the use of SNV WGS to pinpoint a likely site of human infection and identify SNV simultaneously in multiple rodent species in an area of known host-to-human transmission. These results will impact epidemiology and infection control for hantaviruses by tracing zoonotic transmission and investigating possible novel host reservoirs. IMPORTANCE Orthohantaviruses cause severe disease in humans and can be lethal in up to 40% of cases. Sin Nombre orthohantavirus (SNV) is the main cause of hantavirus disease in North America. In this study, we sequenced SNV from an infected patient and wild-caught rodents to trace the location of infection. We also discovered SNV in rodent species not previously known to carry SNV. These studies demonstrate for the first time the use of virus sequencing to trace the transmission of SNV and describe infection in novel rodent species.
Databáze:	MEDLINE
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