Eggshell Derived Europium Doped Hydroxyapatite Nanoparticles for Cell Imaging Application.

Autor: Krishnapriya TK; NEST & NPOED Lab, Department of Physics, Cochin University of Science and Technology, Kochi, 682022, India., Deepti A; Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Kochi, 682022, India., Chakrapani PSB; Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Kochi, 682022, India.; Centre of Excellence in Advanced Materials, Cochin University of Science and Technology, Kochi, 682022, India., Asha AS; NEST & NPOED Lab, Department of Physics, Cochin University of Science and Technology, Kochi, 682022, India. asa@cusat.ac.in.; Centre of Excellence in Advanced Materials, Cochin University of Science and Technology, Kochi, 682022, India. asa@cusat.ac.in., Jayaraj MK; University of Calicut, Malappuram, 673635, India.
Jazyk: angličtina
Zdroj: Journal of fluorescence [J Fluoresc] 2021 Nov; Vol. 31 (6), pp. 1927-1936. Date of Electronic Publication: 2021 Sep 21.
DOI: 10.1007/s10895-021-02814-0
Abstrakt: Hen's eggshell, a biological waste product, was turned into a cell imaging probe: europium doped hydroxyapatite (HAp: Eu) nanoparticle using hydrothermal method. Luminescence of the synthesized nanoparticle was studied for various doping concentrations of the lanthanide ion europium (Eu 3+ ). Eu doped HAp showed a hexagonal crystal structure and rod-shaped morphology. Well-defined emission peaks of europium, corresponding to the substitution of Eu 3+ at the Ca 2+ (I) site of HAp, were confirmed from the samples' photoluminescence (PL) spectra. Good biocompatibility up to 500 μg/mL of the samples indicates their potential applications in bioimaging. Synthesized nanoparticles were internalized and used for in vitro imaging of the PC12 cells without any surface modification. The materials' use as a potential in vivo imaging agent is proposed from the haemolysis study.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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