Cloning, expression, and characterization of an arabitol dehydrogenase and coupled with NADH oxidase for effective production of L-xylulose.

Autor: Zhu CY; School of Pharmacy, Jiangsu University, Zhenjiang, People's Republic of China., Zhu YH; School of Pharmacy, Jiangsu University, Zhenjiang, People's Republic of China., Zhou HP; School of Pharmacy, Jiangsu University, Zhenjiang, People's Republic of China., Xu YY; School of Pharmacy, Jiangsu University, Zhenjiang, People's Republic of China., Gao J; College of Petroleum and Chemical Engineering, Beibu Gulf University, Qinzhou, People's Republic of China., Zhang YW; School of Pharmacy, Jiangsu University, Zhenjiang, People's Republic of China.
Jazyk: angličtina
Zdroj: Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2022; Vol. 52 (5), pp. 590-597. Date of Electronic Publication: 2021 Sep 16.
DOI: 10.1080/10826068.2021.1975299
Abstrakt: A novel arabitol dehydrogenase (ArDH) gene was cloned from a bacterium named Aspergillus nidulans and expressed heterologously in Escherichia coli . The purified ArDH exhibited the maximal activity in pH 9.5 Tris-HCl buffer at 40 °C, showed K m and V max of 1.2 mg/mL and 9.1 U/mg, respectively. The ArDH was used to produce the L-xylulose and coupled with the NADH oxidase (Nox) for the regeneration of NAD + . In further optimization, a high conversion of 84.6% in 8 hours was achieved under the optimal conditions: 20 mM of xylitol, 100 µM NAD + in pH 9.0 Tris-HCl buffer at 30 °C. The results indicated the coupling system with cofactor regeneration provides a promising approach for L-xylulose production from xylitol.
Databáze: MEDLINE
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