CRISPR/Cas9 small promoter deletion in H19 lncRNA is associated with altered cell morphology and proliferation.

Autor: da Silva Santos R; Department of Physiology and Pharmacology, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Brazil., Pinheiro DP; Department of Physiology and Pharmacology, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Brazil., Teixeira LPR; Experimental Biology Nucleus, University of Fortaleza, Fortaleza, Ceará, Brazil., Sales SLA; Department of Physiology and Pharmacology, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Brazil., Dos Santos Luciano MC; Department of Physiology and Pharmacology, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Brazil., de Lima Melo MM; Department of Clinical Medicine, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Brazil., Pinheiro RF; Department of Clinical Medicine, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Brazil., Tavares KCS; Experimental Biology Nucleus, University of Fortaleza, Fortaleza, Ceará, Brazil., Furtado GP; Sector of Biotechnology, Oswaldo Cruz Foundation, FIOCRUZ-Ceará, Fortaleza, Brazil., Pessoa C; Department of Physiology and Pharmacology, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Brazil., Furtado CLM; Drug Research and Development Center, Postgraduate Program in Translational Medicine, Federal University of Ceará, Fortaleza, Brazil. clibardim@gmail.com.; Department of Gynecology and Obstetrics, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Prêto, SP, Brazil. clibardim@gmail.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Sep 15; Vol. 11 (1), pp. 18380. Date of Electronic Publication: 2021 Sep 15.
DOI: 10.1038/s41598-021-97058-0
Abstrakt: The imprinted H19 long non-coding RNA, a knowing oncofetal gene, presents a controversial role during the carcinogenesis process since its tumor suppressor or oncogenic activity is not completely elucidated. Since H19 lncRNA is involved in many biological pathways related to tumorigenesis, we sought to develop a non-cancer lineage with CRISPR-Cas9-mediated H19 knockdown (H19-) and observe the changes in a cellular context. To edit the promoter region of H19, two RNA guides were designed, and the murine C2C12 myoblast cells were transfected. H19 deletion was determined by DNA sequencing and gene expression by qPCR. We observed a small deletion (~ 60 bp) in the promoter region that presented four predicted transcription binding sites. The deletion reduced H19 expression (30%) and resulted in increased proliferative activity, altered morphological patterns including cell size and intracellular granularity, without changes in viability. The increased proliferation rate in the H19- cell seems to facilitate chromosomal abnormalities. The H19- myoblast presented characteristics similar to cancer cells, therefore the H19 lncRNA may be an important gene during the initiation of the tumorigenic process. Due to CRISPR/Cas9 permanent edition, the C2C12 H19- knockdown cells allows functional studies of H19 roles in tumorigenesis, prognosis, metastases, as well as drug resistance and targeted therapy.
(© 2021. The Author(s).)
Databáze: MEDLINE
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