Phenotypic and molecular identification of Brucella suis biotype 1 in a pig from Brazil-case report.

Autor: Vicente AF; Department of Animal Production and Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Zootechnics, State University of Sao Paulo, Botucatu, SP, Brazil., Mioni MSR; Department of Animal Production and Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Zootechnics, State University of Sao Paulo, Botucatu, SP, Brazil., Cagnini DQ; Department of Veterinary Clinical Science, Federal University of Jataí, Jataí, GO, Brazil., Ribeiro MG; Department of Animal Production and Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Zootechnics, State University of Sao Paulo, Botucatu, SP, Brazil., Arabe Filho MF; Department of Animal Production and Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Zootechnics, State University of Sao Paulo, Botucatu, SP, Brazil., Listoni FJP; Department of Animal Production and Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Zootechnics, State University of Sao Paulo, Botucatu, SP, Brazil., Ribeiro BLD; Department of Animal Production and Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Zootechnics, State University of Sao Paulo, Botucatu, SP, Brazil., Megid J; Department of Animal Production and Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Zootechnics, State University of Sao Paulo, Botucatu, SP, Brazil. jane.megid@unesp.br.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2022 Mar; Vol. 53 (1), pp. 487-489. Date of Electronic Publication: 2021 Sep 11.
DOI: 10.1007/s42770-021-00607-y
Abstrakt: Brucellosis is a zoonotic disease with a global impact. Brucella suis is one of the most pathogenic species to humans, requiring different measures for the control and/or eradication of the disease. The serological investigation for brucellosis was performed in pigs, horses, dogs, and cattle on a farm with a history of abortion in sows and necropsy of a boar with severe necrosuppurative orchitis. One sow, two cows, and two dogs reveled positive to Rose Bengal Test (RBT), although only the sow had a confirmatory outcome in 2-mercaptoethanol (2-ME). The 2-ME-positive sow was euthanized and microbiological culture of lymph nodes and liver followed by biochemical characterization allowed phenotypic characterization of Brucella suis biotype 1. PCR multiplex Bruce-ladder and Suis-ladder enabled molecular confirmation, respectively, of Brucella suis and biotype 1. The transmission aspects of B. suis to pigs and other domestic species, the combination of diagnostic procedures to diagnosis, as well as human health concerns of brucellosis are discussed.
(© 2021. Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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