Phytochemical Study and Antioxidant Activities of the Water-Soluble Aerial Parts and Isolated Compounds of Thymus munbyanus subsp. ciliatus (Desf.) Greuter & Burdet

Autor: Chaouche M; Mentouri Constantine Valorization of Natural Resources University, Department of Chemistry, Bioactive Molecules and Biological Analysis Unit, Constantine, Algeria, Demirtaş İ; Iğdır University Faculty of Arts and Sciences, Department of Biochemistry, Iğdır, Turkey, Koldaş S; Çankırı Karatekin University Faculty of Science, Department of Chemistry, Çankırı, Turkey, Tüfekçi AR; Çankırı Karatekin University Faculty of Science, Department of Chemistry, Çankırı, Turkey, Gül F; Iğdır University Faculty of Arts and Sciences, Department of Biochemistry, Iğdır, Turkey, Özen T; Ondokuz Mayıs University Faculty of Arts and Sciences, Department of Chemistry, Samsun, Turkey, Wafa N; Ferhat Abbas Setif University Faculty of Natural Life and Sciences, Laboratory of Phytotherapy Applied to Chronic Diseases, El Bez, Algeria, Boureghda A; Mentouri Constantine Valorization of Natural Resources University, Department of Chemistry, Bioactive Molecules and Biological Analysis Unit, Constantine, Algeria, Bora N; Ondokuz Mayıs University Faculty of Arts and Sciences, Department of Chemistry, Samsun, Turkey
Jazyk: angličtina
Zdroj: Turkish journal of pharmaceutical sciences [Turk J Pharm Sci] 2021 Sep 01; Vol. 18 (4), pp. 430-437.
DOI: 10.4274/tjps.galenos.2020.44538
Abstrakt: Objectives: The present study aimed to determine the phenolic compounds present in the water-soluble extracts of Thymus munbyanus subsp. ciliatus using high pressure liquid chromatography-time-of-flight mass spectrometry (MS). These phenolic compounds were further isolated and characterized for their antioxidant activities.
Materials and Methods: The aerial parts of T. munbyanus subsp. ciliatus were air dried, powdered, and extracted using water:methanol three times. The concentrated hydromethanolic extract was further dissolved in H 2 O, filtered, and successively extracted using ethyl acetate, chloroform, and n -butanol. T. munbyanus extracts were further purified using column chromatography, and the purified extracts were subjected to in vitro antioxidant assays.
Results: Two previously undescribed compounds, namely methyl 2,3,5,6-tetrahydroxybenzoate and 4-hydroxy-5-methoxy-2-oxo-2H-pyran-3-carboxylic acid, and 14 known compounds, including 3 flavonoids; namely 3',5,5',7-tetrahydroxyflavanone, luteolin, and isorhamnetin-3-O-β-glucoside; a sterol glucoside named daucosterol; and 10 phenolic compounds, namely salicylic acid, ferulic acid, pluchoic acid, ethyl caffeate, methyl caffeate, protocatechuic acid, rosmarinic acid, p-coumaric acid, tyrosol, and protocatechuic aldehyde, were isolated from ethyl acetate and n -butanol extracts. The isolated compounds were characterized using 1D-2D -1 H -13 C nuclear magnetic resonance and MS methods.
Conclusion: The compounds isolated from ethyl acetate and n-butanol extracts exhibited excellent antioxidant and 2,2-diphenyl-1-picrylhydrazyl scavenging activities. All these results highlighted the antioxidant potential of the isolated phenolic compounds and extracts, which could be further utilized for different pharmacological applications.
Databáze: MEDLINE
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