Near-infrared Transillumination and Photodynamic Therapy Using Hypericin in Animal Laryngeal Tumors.

Autor: Lee H; Department of Thoracic and Cardiovascular Surgery, Kosin University College of Medicine, Busan, 49267, Korea., Kim SW; Department of Head and Neck Surgery and Otolaryngology, Kosin University College of Medicine, Busan, 49267, Korea.; Kosin Innovative Smart Healthcare Research Center, Kosin University Gospel Hospital, Busan, 49267, Korea., Kwon DY; Kosin Innovative Smart Healthcare Research Center, Kosin University Gospel Hospital, Busan, 49267, Korea.; Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology, Pukyong National University, Busan, 48513, Korea., Kang HW; Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology, Pukyong National University, Busan, 48513, Korea., Jung MJ; Department of Pathology, Kosin University College of Medicine, Busan, 49267, Korea., Kim JH; Department of Internal Medicine, Kosin University College of Medicine, Busan, 49267, Korea., Ahn YC; Kosin Innovative Smart Healthcare Research Center, Kosin University Gospel Hospital, Busan, 49267, Korea. ahny@pknu.ac.kr.; Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology, Pukyong National University, Busan, 48513, Korea. ahny@pknu.ac.kr., Oak C; Kosin Innovative Smart Healthcare Research Center, Kosin University Gospel Hospital, Busan, 49267, Korea. oaks70@daum.net.; Department of Internal Medicine, Kosin University College of Medicine, Busan, 49267, Korea. oaks70@daum.net.
Jazyk: angličtina
Zdroj: Tissue engineering and regenerative medicine [Tissue Eng Regen Med] 2021 Dec; Vol. 18 (6), pp. 941-951. Date of Electronic Publication: 2021 Sep 08.
DOI: 10.1007/s13770-021-00377-6
Abstrakt: Background: We aimed to validate a pilot study of photodiagnosis using near infrared (NIR) transillumination and assess the clinical efficacy of hypericin-mediated photodynamic therapy (HYP-PDT) in a rabbit laryngeal cancer model in order to develop a novel therapeutic modality with complete remission and preservation of the functional organ.
Methods: (1) In vitro study: VX tumor cells were subcultured and subjected to HYP-PDT. (2) In vivo study: A laryngeal cancer model was developed in which 12 rabbits were inoculated with a VX tumor suspension in the submucosal area of the left vocal fold using a transoral approach. All rabbits underwent NIR transillumination using light with a wavelength of 780 nm. The survival periods of the three treatment groups (6 rabbits in Group A: HYP-PDT, 3 each in Groups B and C: laser irradiation or HYP administration only) were analyzed.
Results: The higher the HYP concentration, the lower the VX cell viability in response to HYP-PDT using 590 nm LED. Following HYP-PDT, small tumors in Group A-1 rabbits healed completely and the animals demonstrated a long survival period, and larger tumors in Group A-2 healed partially with a survival period that extended over 3 weeks after inoculation. The survival of Groups B and C were not different over the first 3 weeks of the study, and were shorter than in Group A.
Conclusion: We found HYP-PDT could be a curative therapy for early-stage cancers that may also preserve organ function, and may inhibit tumor progression and metastasis during advanced stages of laryngeal cancer.
(© 2021. The Korean Tissue Engineering and Regenerative Medicine Society.)
Databáze: MEDLINE
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