A Defined Antigen Skin Test for Diagnosis of Bovine Tuberculosis in Domestic Water Buffaloes ( Bubalus bubalis ).

Autor:	Kumar T; College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India., Singh M; College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India., Jangir BL; Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India., Arora D; Haryana Pashu Vigyan Kendra, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India., Srinivasan S; Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, United States., Bidhan D; Department of Livestock Production Management, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India., Yadav DC; Department of Livestock Production Management, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India., Veerasami M; Cisgen Biotech Discoveries Pvt. Ltd., Chennai, India., Bakker D; Independent Researcher, Lelystad, Netherlands., Kapur V; Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, United States.; Department of Animal Science, The Pennsylvania State University, University Park, PA, United States., Jindal N; Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2021 Aug 16; Vol. 8, pp. 669898. Date of Electronic Publication: 2021 Aug 16 (Print Publication: 2021).
DOI:	10.3389/fvets.2021.669898
Abstrakt:	Bovine tuberculosis (bTB) remains endemic in domestic water buffaloes ( Bubalus bubalis ) in India and elsewhere, with limited options for control other than testing and slaughter. The prescribed tuberculin skin tests with purified protein derivative (PPD) for diagnosis of bTB preclude the use of Bacille Calmette-Guérin (BCG)-based vaccination because of the antigenic cross-reactivity of vaccine strains with Mycobacterium bovis and related pathogenic members of the M. tuberculosis complex (MTBC). For the diagnosis of bTB in domestic water buffaloes, we here assessed a recently described defined-antigen skin test (DST) that comprises overlapping peptides representing the ESAT-6, CFP-10 and Rv3615c antigens, present in disease-causing members of the MTBC but missing in BCG strains. The performance characteristics of three doses (5, 10 or 20 μg/peptide) of the DST were assessed in natural tuberculin skin test reactor ( n = 11) and non-reactor ( n = 35) water buffaloes at an organized dairy farm in Hisar, India, and results were compared with the single intradermal skin test (SIT) using standard bovine tuberculin (PPD-B). The results showed a dose-dependent response of DST in natural reactor water buffaloes, although the SIT induced a significantly greater ( P < 0.001) skin test response than the highest dose of DST used. However, using a cut-off of 2 mm or greater, the 5, 10, and 20 μg DST cocktail correctly classified eight, 10 and all 11 of the SIT-positive reactors, respectively, suggesting that the 20 μg DST cocktail has a diagnostic sensitivity (Se) of 1.0 (95% CI: 0.72-1.0) identical to that of the SIT. Importantly, none of the tested DST doses induced any measurable skin induration responses in the 35 SIT-negative animals, suggesting a specificity point estimate of 1.0 (95% CI: 0.9-1.0), also identical to that of the SIT and compares favorably with that of the comparative cervical test (Se = 0.85; 95% CI: 0.55-0.98). Overall, the results suggest that similar to tuberculin, the DST enables sensitive and specific diagnosis of bTB in water buffaloes. Future field trials to explore the utility of DST as a defined antigen replacement for tuberculin in routine surveillance programs and to enable BCG vaccination of water buffaloes are warranted. Competing Interests: MV was employed by company Cisgen Biotech Discoveries Pvt. Ltd. SS and VK have filed intellectual property protection for the DST. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Kumar, Singh, Jangir, Arora, Srinivasan, Bidhan, Yadav, Veerasami, Bakker, Kapur and Jindal.)
Databáze:	MEDLINE
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