Visnagin prevents isoproterenol-induced myocardial injury by attenuating oxidative stress and inflammation and upregulating Nrf2 signaling in rats.

Autor: Abukhalil MH; Department of Biology, Faculty of Science, Al-Hussein Bin Talal University, Ma'an, Jordan.; Department of Medical Analysis, Princess Aisha Bint Al-Hussein Faculty of Nursing and Health Sciences, Al-Hussein Bin Talal University, Ma'an, Jordan., Hussein OE; Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt., Aladaileh SH; Department of Medical Analysis, Princess Aisha Bint Al-Hussein Faculty of Nursing and Health Sciences, Al-Hussein Bin Talal University, Ma'an, Jordan.; Department of Pharmacy Practice, College of Pharmacy, University of Hafr Al-Batin, Hafr Al-Batin, Saudi Arabia., Althunibat OY; Department of Medical Analysis, Princess Aisha Bint Al-Hussein Faculty of Nursing and Health Sciences, Al-Hussein Bin Talal University, Ma'an, Jordan., Al-Amarat W; Department of Medical Support, Al-karak University College, Al-Balqa' Applied University, As-Salt, Jordan., Saghir SA; Department of Medical Analysis, Princess Aisha Bint Al-Hussein Faculty of Nursing and Health Sciences, Al-Hussein Bin Talal University, Ma'an, Jordan., Alfwuaires MA; Department of Biological Sciences, Faculty of Science, King Faisal University, Al-Ahsa, Saudi Arabia., Algefare AI; Department of Biological Sciences, Faculty of Science, King Faisal University, Al-Ahsa, Saudi Arabia., Alanazi KM; Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia., Al-Swailmi FK; Department of Pharmacy Practice, College of Pharmacy, University of Hafr Al-Batin, Hafr Al-Batin, Saudi Arabia., Kamel EM; Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt., Mahmoud AM; Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.; Biotechnology Department, Research Institute of Medicinal and Aromatic Plants, Beni-Suef University, Beni-Suef, Egypt.
Jazyk: angličtina
Zdroj: Journal of biochemical and molecular toxicology [J Biochem Mol Toxicol] 2021 Nov; Vol. 35 (11), pp. e22906. Date of Electronic Publication: 2021 Sep 05.
DOI: 10.1002/jbt.22906
Abstrakt: Oxidative tissue injury and inflammatory responses play major roles in cardiovascular diseases and heart failure. Visnagin (VIS) is a natural bioactive component of Ammi visnaga, with promising radical scavenging and anti-inflammatory activities. This study explored the protective effect of VIS against isoproterenol (ISO)-induced acute myocardial injury and oxidative stress in rats. VIS was supplemented for 14 days, and the rats received ISO (100 mg/kg) twice at an interval of 24 h. ISO-induced myocardial injury was characterized by elevated serum CK-MB, LDH, and troponin-I associated with increased heart weight and several histopathological changes. ISO increased reactive oxygen species (ROS), malondialdehyde (MDA), NF-κB p65, TNF-α, IL-6, and decreased glutathione and antioxidant enzymes in rats' hearts. VIS prevented myocardial injury and ameliorated the cardiac function markers, ROS, MDA, NF-κB p65, and pro-inflammatory cytokines in ISO-intoxicated rats. In addition, VIS decreased Bax mRNA and caspases, and upregulated Nrf2, HO-1, Bcl-2, and PPARγ. Molecular docking simulations revealed the binding method of VIS to NF-κB, Keap1, and PPARγ. In conclusion, VIS protects against ISO-induced acute myocardial injury by attenuating oxidative tissue injury and reducing key inflammatory and apoptosis markers. In vivo and in silico results showed that activation of Nrf2/HO-1 signaling and PPARγ mediates the cardioprotective effect of VIS.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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