Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo.

Autor: Bastos NM; São Paulo State University (UNESP), Department of Animal Reproduction, Graduate Program in Genetics and Animal Breeding, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, Brazil. Electronic address: nataliamarinsbastos@yahoo.com.br., Rossi GF; São Paulo State University (UNESP), Department of Animal Reproduction, Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, Brazil., da Silva Leão BC; São Paulo State University (UNESP), Department of Animal Reproduction, Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, Brazil., Negrão F; Proteomics Center of Excellence, Northwestern University, 2170 Campus Dr, Evanston, IL, USA; ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas, Cidade Universitaária Zeferino Vaz, Campinas, São Paulo, Brazil., Ferreira CR; ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas, Cidade Universitaária Zeferino Vaz, Campinas, São Paulo, Brazil; Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA., Vrisman DP; São Paulo State University (UNESP), Department of Animal Reproduction, Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, Brazil., Rodrigues NN; São Paulo State University (UNESP), Department of Animal Reproduction, Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, Brazil., Zorzetto MF; Instituto de Zootecnia (IZ/APTA), Sertãozinho, São Paulo, Brazil., de Paula Freitas-Dell'Aqua C; São Paulo State University (UNESP), School of Veterinary Medicine and Animal Science, Campus Botucatu, São Paulo, Brazil., Vantini R; São Paulo State University (UNESP), Department of Animal Reproduction, Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, Brazil., Monteiro FM; São Paulo State University (UNESP), Department of Animal Reproduction, Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, Brazil; Instituto de Zootecnia (IZ/APTA), Sertãozinho, São Paulo, Brazil., Mercadante MEZ; Instituto de Zootecnia (IZ/APTA), Sertãozinho, São Paulo, Brazil., Eberlin MN; ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas, Cidade Universitaária Zeferino Vaz, Campinas, São Paulo, Brazil., de Lima VFMH; São Paulo State University (UNESP), Department of Animal Reproduction, Graduate Program in Genetics and Animal Breeding, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, Brazil., Mingoti GZ; São Paulo State University (UNESP), Department of Animal Reproduction, Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, Brazil; São Paulo State University (UNESP), School of Veterinary Medicine, Laboratory of Reproductive Physiology, Campus Araçatuba, São Paulo, Brazil. Electronic address: gisele.mingoti@unesp.br.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2021 Nov; Vol. 175, pp. 23-33. Date of Electronic Publication: 2021 Aug 26.
DOI: 10.1016/j.theriogenology.2021.08.031
Abstrakt: The aim of this study was to examine the effects of long-term dietary supplementation of young Nellore bulls with rumen-protected polyunsaturated fatty acids (PUFAs) and of the inclusion of catalase in the semen extender on semen quality, in vitro sperm fertilizing ability, and intracytoplasmic lipid content in the resulting embryos. Twelve Nellore bulls were supplemented with rumen-protected PUFAs or with a basal diet from 14 to 24 months of age. The semen was collected at the end of supplementation. For cryopreservation, the ejaculate was divided into two equal volumes and catalase was added to the extender in one of the fractions. Thus, the experimental design consisted of a 2 × 2 factorial scheme with two diets (control and PUFA) and two extenders (Cat+ and Cat-). Total motility and the percentage of rapid cells in fresh semen were negatively affected by dietary supplementation with PUFAs (P < 0.05), but these effects did not persist after freezing. The frozen/thawed semen of animals fed PUFAs exhibited an increase in the percentages of damaged plasma and acrosomal membranes, as well as an increase in the proportion of lipids ions at m/z 578 and m/z 757 detected by MALDI-MS. Nevertheless, there was no effect of the treatments on in vitro embryo development. However, embryos derived from bulls supplemented with PUFAs exhibited higher lipid accumulation compared to control (P < 0.05). In conclusion, PUFA supplementation promoted worsening of semen quality without affecting the in vitro sperm fertilizing ability; however, the paternal diet affected the intracytoplasmic lipid content in the resulting embryos.
Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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