Neuroprotective flavonoids of the leaf of Antiaris africana Englea against cyanide toxicity.

Autor: Ilesanmi OB; Department of Biochemistry, Faculty of Science, The Federal University, Otuoke, Bayelsa State PMB 126, Yenagoa, Bayelsa State, Nigeria. Electronic address: ilesanmiob@fuotuoke.edu.ng., Akinmoladun AC; Department of Biochemistry, School of Life Sciences, The Federal University of Technology, PMB 704, Akure, Nigeria., Elusiyan CA; Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife, Nigeria., Ogungbe IV; Department of Chemistry, Physics, and Atmospheric Sciences, Jackson State University, USA., Olugbade TA; Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife, Nigeria., Olaleye MT; Department of Biochemistry, School of Life Sciences, The Federal University of Technology, PMB 704, Akure, Nigeria.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2022 Jan 10; Vol. 282, pp. 114592. Date of Electronic Publication: 2021 Sep 01.
DOI: 10.1016/j.jep.2021.114592
Abstrakt: Ethnopharmacological Relevance: Different parts of Antiaris africana Englea (Moraceae) are used traditionally for the treatment of various diseases, including epilepsy and other nervous system disorders.
Aims of This Study: The current study was designed to evaluate the neuroprotective activity of flavonoids isolated from A. africana against potassium cyanide (KCN)-induced oxidative damage in brain homogenate.
Materials and Methods: Dried and ground leaves of A. africana were extracted with methanol and fractioned into n-hexane (HFA), dichloromethane (DFA), ethyl acetate (EFA) and methanol (MFA). Each fraction was assessed for neuroprotective potential by anticholinesterase activity test. The fraction with the best anticholinesterase activity was subjected to various chromatographic techniques through bioassay-guided fractionation to isolate the bioactive compounds. The protective ability of the extract, fractions and compounds against Potassium cyanide (KCN)-induced mitochondrial damage in rat brain homogenate was evaluated. Structures of the isolated compounds were determined using 1D and 2D NMR, mass spectrometry and by comparison with literature data.
Results and Discussion: The ethyl acetate fraction showed the best anticholinesterase activity with an IC 50 of 23.23 ± 1.12 μg/ml. Quercetin and a biflavonoid glucoside identified as 3'-4'-bisquercetin-3β-D-diglucoside from this fraction displayed a remarkable antioxidant activity in the DPPH assay and showed significant (P < 0.05) increase in the activity of dehydrogenase inhibited by KCN in a concentration dependent manner. However, quercetin was more effective in reducing the MDA level and acetylcholinesterase activity that were elevated by KCN.
Conclusion: Quercetin and the bisquercetin-diglucoside isolated from the leaves of A. Africana for the first time, are major contributors to the observed neuroprotective property of the plant which supports its folkloric usage in the management of seizures, epilepsy and other neurological disorders.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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