| Autor: |
Garcia KD; Laboratory Animal Medicine, IIT-IITRI, Chicago, Illinois., Coda KA; Research Animal Resources, University of Minnesota, Minneapolis, Minnesota., Smith AA; Abbvie, Abbott Park, Illinois., Condren AR; Department of Pharmaceutical Sciences, University of Illinois at Chicago, Chicago, Illinois., Deng Y; Department of Statistics, University of Illinois at Urbana-Champaign, Champaign, Illinois., Perkins C; Charles River, Wilmington, Massachusetts., Sanchez LM; Department of Chemistry and Biochemistry, University of California at Santa Cruz, California., Fortman JD; Biologic Resources Laboratory, University of Illinois at Chicago, Chicago, Illinois. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of the American Association for Laboratory Animal Science : JAALAS [J Am Assoc Lab Anim Sci] 2021 Nov 01; Vol. 60 (6), pp. 655-660. Date of Electronic Publication: 2021 Sep 01. |
| DOI: |
10.30802/AALAS-JAALAS-21-000004 |
| Abstrakt: |
The number of zebrafish in biomedical research has increased exponentially over the past decades, leading to pressure on the laboratory animal community to develop and refine techniques to monitor zebrafish health so that suitable stocks can be maintained for research. The water filtration assay is a promising technique in which water from a zebrafish system is filtered, and the filter analyzed by PCR. In the present report, we studied how the volume of water tested and the concentration of bacterial pathogens affected test results. To do so, we used stock solutions of 3 zebrafish pathogens: Edwardsiella ictaluri , Aeromonas hydrophila , and Mycobacterium marinum . We used these stocks to create solutions with known concentrations of each pathogen, ranging between 10² and 10 7 Colony Forming Units (CFU) per ml. One, 2, and 3 L of each solution was filtered using positive pressure, and the filters were submitted to a commercial lab for PCR testing. Results were fit with a logistic regression model, and the probability of obtaining a positive result were calculated. Test sensitivity varied by organism, but in general, test results were positively correlated with the volume of the water filtered and with the concentration of bacteria in solution. We conclude that a positive result can be expected for E. ictaluri at 10 5 CFU per mL, A. hydrophila at 10 6 CFU per ml, and M. marinum at 10 6 CFU per mL, when 3 L of solution are filtered. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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