Campylobacter jejuni genotypes are associated with post-infection irritable bowel syndrome in humans.

Autor:	Peters S; Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA., Pascoe B; The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK., Wu Z; Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA., Bayliss SC; The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK., Zeng X; Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA., Edwinson A; Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA., Veerabadhran-Gurunathan S; Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA., Jawahir S; Minnesota Department of Health, St. Paul, MN, USA., Calland JK; The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK., Mourkas E; The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK., Patel R; Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA., Wiens T; Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA., Decuir M; Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA., Boxrud D; Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA., Smith K; Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA., Parker CT; United States Department of Agriculture, Albany, CA, USA., Farrugia G; Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA., Zhang Q; Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA., Sheppard SK; The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK. s.k.sheppard@bath.ac.uk., Grover M; Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA. Grover.Madhusudan@mayo.edu.
Jazyk:	angličtina
Zdroj:	Communications biology [Commun Biol] 2021 Aug 30; Vol. 4 (1), pp. 1015. Date of Electronic Publication: 2021 Aug 30.
DOI:	10.1038/s42003-021-02554-8
Abstrakt:	Campylobacter enterocolitis may lead to post-infection irritable bowel syndrome (PI-IBS) and while some C. jejuni strains are more likely than others to cause human disease, genomic and virulence characteristics promoting PI-IBS development remain uncharacterized. We combined pangenome-wide association studies and phenotypic assays to compare C. jejuni isolates from patients who developed PI-IBS with those who did not. We show that variation in bacterial stress response (Cj0145_phoX), adhesion protein (Cj0628_CapA), and core biosynthetic pathway genes (biotin: Cj0308_bioD; purine: Cj0514_purQ; isoprenoid: Cj0894c_ispH) were associated with PI-IBS development. In vitro assays demonstrated greater adhesion, invasion, IL-8 and TNFα secretion on colonocytes with PI-IBS compared to PI-no-IBS strains. A risk-score for PI-IBS development was generated using 22 genomic markers, four of which were from Cj1631c, a putative heme oxidase gene linked to virulence. Our finding that specific Campylobacter genotypes confer greater in vitro virulence and increased risk of PI-IBS has potential to improve understanding of the complex host-pathogen interactions underlying this condition. (© 2021. The Author(s).)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu Plný text ve formátu PDF