Establishment of an Intradermal Ear Injection Model of IL-17A and IL-36γ as a Tool to Investigate the Psoriatic Cytokine Network.

Autor: Kluwig D; Institute of Biochemistry and Molecular Biology, RWTH Aachen University, 52074 Aachen, Germany.; Department of Dermatology and Allergology, Faculty of Medicine, RWTH Aachen University, 52074 Aachen, Germany., Huth S; Department of Dermatology and Allergology, Faculty of Medicine, RWTH Aachen University, 52074 Aachen, Germany., Abdallah AT; Interdisciplinary Center for Clinical Research (IZKF), Faculty of Medicine, RWTH Aachen University, 52074 Aachen, Germany., Pfaff CM; Institute of Biochemistry and Molecular Biology, RWTH Aachen University, 52074 Aachen, Germany.; Department of Dermatology and Allergology, Faculty of Medicine, RWTH Aachen University, 52074 Aachen, Germany., Fietkau K; Department of Dermatology and Allergology, Faculty of Medicine, RWTH Aachen University, 52074 Aachen, Germany., Huth L; Department of Dermatology and Allergology, Faculty of Medicine, RWTH Aachen University, 52074 Aachen, Germany., Marquardt Y; Department of Dermatology and Allergology, Faculty of Medicine, RWTH Aachen University, 52074 Aachen, Germany., Baron JM; Department of Dermatology and Allergology, Faculty of Medicine, RWTH Aachen University, 52074 Aachen, Germany., Lüscher B; Institute of Biochemistry and Molecular Biology, RWTH Aachen University, 52074 Aachen, Germany.
Jazyk: angličtina
Zdroj: Life (Basel, Switzerland) [Life (Basel)] 2021 Aug 19; Vol. 11 (8). Date of Electronic Publication: 2021 Aug 19.
DOI: 10.3390/life11080846
Abstrakt: Psoriasis is a chronic skin disease affecting 2-3% of the global population. The proinflammatory IL-17A is a key cytokine in psoriasis. Accumulating evidence has revealed that IL-36γ plays also a pathogenic role. To understand more precisely the role of the IL-17A-IL-36γ cytokine network in skin pathology, we used an ear injection model. We injected IL-17A or IL-36γ alone and in combination into the ear pinnae of mice. This resulted in a significant increase in ear thickness measured over time. Histological evaluation of IL-17A + IL-36γ-treated skin showed a strong acanthosis, hyperparakeratosis and infiltration of neutrophils. The same histological features were found in mice after injection of IL-36γ alone, but to a lesser extent. IL-17A alone was not able to induce psoriasis-like changes. Genes encoding proteins of the S100 family, antimicrobial peptides and chemo-attractants for neutrophils were upregulated in the IL-17A + IL-36γ group. A much weaker expression was seen after the injection of each cytokine alone. These results strengthen the hypothesis that IL-17A and IL-36γ drive psoriatic inflammation via a synergistic interaction. Our established intradermal ear injection model can be utilized in the future to monitor effects of various inhibitors of this cytokine network.
Databáze: MEDLINE
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