| Autor: |
Rozolen JM; Department of Veterinary Surgery and Animal Reproduction, Sao Paulo State University-UNESP, Botucatu 18618-681, Brazil., Teodoro TGW; Department of Veterinary Clinic, Sao Paulo State University-UNESP, Botucatu 18618-681, Brazil., Sobral RA; ONCO CANE-Veterinary, São Paulo 04084-002, Brazil., Sueiro FAR; VetPat Veterinary Laboratory, Campinas 13073-022, Brazil., Laufer-Amorim R; Department of Veterinary Clinic, Sao Paulo State University-UNESP, Botucatu 18618-681, Brazil., Elias F; Veterinary School, Federal University of Fronteira Sul-UFFS, Realeza 85770-000, Brazil., Fonseca-Alves CE; Department of Veterinary Surgery and Animal Reproduction, Sao Paulo State University-UNESP, Botucatu 18618-681, Brazil.; Institute of Health Sciences, Paulista University-UNIP, Bauru 17048-290, Brazil. |
| Abstrakt: |
Splenic hemangiosarcoma (HSA) is a malignant tumor of endothelial cells that affects middle-aged and elderly dogs and is characterized by the formation of new blood vessels, commonly associated with necrotic and hemorrhagic areas. Despite its importance in veterinary medicine, few studies have identified markers with prognostic value for canine HSA. Thus, this study aimed to associate the clinicopathological findings (prostate-specific membrane antigen [PSMA], Claudin-5, and Ki67 gene and protein expression) with overall survival in HSA-affected patients. Fifty-three formalin-fixed and paraffin-embedded canine splenic HSA samples, previously diagnosed by histopathological examination, were used in this study. Claudin-5, PSMA, and Ki67 protein expression levels were evaluated by immunohistochemistry, and gene expression was evaluated by quantitative polymerase chain reaction. Claudin-5 protein overexpression was observed in patients with metastasis ( p = 0.0078) and with stage III tumors compared to those with stage I and II tumors ( p = 0.0451). In patients treated with surgery alone, low PSMA gene and protein expression ( p = 0.05 and p = 0.0355, respectively) were associated with longer survival time. Longer survival time was observed in patients with a low Ki67 index ( p = 0.0488). Our results indicate that Claudin-5 protein expression is associated with metastatic status, and PSMA gene and protein expression, and Ki67 index are associated with survival time. |
