Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings.

Autor:	Liu L; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA.; Department of Pediatric Otolaryngology, Nationwide Children's Hospital, Columbus, OH 43205, USA., Dharmadhikari S; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA.; Department of Pediatric Otolaryngology, Nationwide Children's Hospital, Columbus, OH 43205, USA., Pouliot RA; Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405, USA., Li MM; Department of Pediatric Otolaryngology, Nationwide Children's Hospital, Columbus, OH 43205, USA., Minneci PM; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA., Tan Z; College of Medicine, The Ohio State University, Columbus, OH 43210, USA., Shontz K; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA., Johnson J; Nanofiber Solutions, Inc., Columbus, OH 43017, USA., Reynolds SD; Center for Perinatal Research, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA., Breuer CK; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA.; Department of Pediatric Surgery, Nationwide Children's Hospital, Columbus, OH 43205, USA., Weiss DJ; Department of Pediatric Otolaryngology, Nationwide Children's Hospital, Columbus, OH 43205, USA., Chiang T; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA.; Department of Pediatric Otolaryngology, Nationwide Children's Hospital, Columbus, OH 43205, USA.
Jazyk:	angličtina
Zdroj:	Bioengineering (Basel, Switzerland) [Bioengineering (Basel)] 2021 Aug 20; Vol. 8 (8). Date of Electronic Publication: 2021 Aug 20.
DOI:	10.3390/bioengineering8080116
Abstrakt:	Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo.
Databáze:	MEDLINE
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