Development of castor polyurethane scaffold ( Ricinus communis L.) and its effect with stem cells for bone repair in an osteoporosis model.

Autor:	Pacheco IKC; Graduate Program in Dentistry, Federal University of Piauí, Teresina, Brasil., Reis FDS; Graduate Program in Materials Science, Federal University of Piauí, Teresina, Brasil., Carvalho CES; Graduate Program in Animal Science, Federal University of Piauí, Teresina, Brasil., De Matos JME; Department of Chemistry, Federal University of Piauí, Teresina, Brasil., Argôlo Neto NM; Department of Veterinary Clinic and Surgery, Federal University of Piauí, Teresina, Brasil., Baeta SAF; Department of Veterinary Clinic and Surgery, Federal University of Piauí, Teresina, Brasil., Silva KRD; Department of Pathology and Dental Clinic, Federal University of Piauí, Teresina, Brasil., Dantas HV; Graduate Program in Dentistry, Federal University of Parnaíba, João Pessoa, Brasil., Sousa FB; Department of Morphology, Federal University of Parnaíba, João Pessoa, Brasil., Fialho ACV; Department of Pathology and Dental Clinic, Federal University of Piauí, Teresina, Brasil.
Jazyk:	angličtina
Zdroj:	Biomedical materials (Bristol, England) [Biomed Mater] 2021 Sep 07; Vol. 16 (6). Date of Electronic Publication: 2021 Sep 07.
DOI:	10.1088/1748-605X/ac1f9e
Abstrakt:	The development of 'smart' scaffolds has achieved notoriety among current prospects for bone repair, especially for chronic osteopathy, such as osteoporosis. Millions of individuals in the world suffer from poor bone healing due to osteoporosis. The objective of this work was to produce and characterize castor polyurethane (PU) scaffolds ( Ricinus communis L.) and evaluate its in vitro biocompatibility with stem cells and osteoinductive effect in vivo on bone failures in a leporid model of osteoporosis. The material was characterized using Fourier-transform infrared spectroscopy, thermogravimetric analysis, SEM, and porosity analysis. Then, the biocompatibility was assessed by adhesion using SEM and cytotoxicity in a 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium assay. The osteoinductive effect in vivo was determined in bone defects in rabbit tibias ( Oryctolagus cuniculus ) submitted to castor PU scaffold, castor PU scaffold associated with stem cells, and negative control, after four and eight weeks, evaluated by computed microtomography and histopathology. The scaffolds were porous, with an average pore size of 209.5 ± 98.2 µm, absence of cytotoxicity, and positive cell adhesiveness in vitro. All the animals presented osteoporosis, characterized by multifocal osteoblastic inactivity and areas of mild fibrosis. There were no statistical differences between these treatments in the fourth week of treatment. In the eighth week, the treatment with castor PU scaffold alone induced more significant bone formation when compared to the other groups, followed by treatment with an association between castor PU scaffold and stem cells. The castor PU scaffold was harmless to cell culture, favoring cell adhesiveness and proliferation, in addition to inducing bone neoformation in osteoporotic rabbits. (© 2021 IOP Publishing Ltd.)
Databáze:	MEDLINE
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