Membrane Bound Peroxiredoxin-1 Serves as a Biomarker for In Vivo Detection of Sessile Serrated Adenomas.

Autor: Jaiswal S; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Joshi B; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Chen J; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Wang F; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Dame MK; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Spence JR; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA., Newsome GM; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Katz EL; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Shah YM; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.; Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA., Ramakrishnan SK; Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA., Li G; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Lee M; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Appelman HD; Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA., Kuick R; Department of Biostatistics, and University of Michigan, Ann Arbor, Michigan, USA., Wang TD; Division of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.; Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Jazyk: angličtina
Zdroj: Antioxidants & redox signaling [Antioxid Redox Signal] 2022 Jan; Vol. 36 (1-3), pp. 39-56. Date of Electronic Publication: 2021 Dec 21.
DOI: 10.1089/ars.2020.8244
Abstrakt: Aim: Sessile serrated adenomas (SSAs) are premalignant lesions driven by the BRAF V600E mutation to give rise to colorectal cancers (CRCs). They are often missed during white light colonoscopy because of their subtle appearance. Previously, a fluorescently labeled 7mer peptide KCCFPAQ was shown to detect SSAs in vivo . We aim to identify the target of this peptide. Results: Peroxiredoxin-1 (Prdx1) was identified as the binding partner of the peptide ligand. In vitro binding assays and immunofluorescence staining of human colon specimens ex vivo supported this result. Prdx1 was overexpressed on the membrane of cells with the BRAF V600E mutation, and this effect was dependent on oxidative stress. RKO cells harboring the BRAF V600E mutation and human SSA specimens showed higher oxidative stress as well as elevated levels of Prdx1 on the cell membrane. Innovation and Conclusion: These results suggest that Prdx1 is overexpressed on the cell surface in the presence of oxidative stress and can serve as an imaging biomarker for in vivo detection of SSAs. Antioxid. Redox Signal . 36, 39-56.
Databáze: MEDLINE
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