| Autor: |
Zhou C; Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, Davis, California 95616, United States., Fang Z; Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, Davis, California 95616, United States.; Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.; Shenzhen Engineering Laboratory of Single-Molecule Detection and Instrument Development, Shenzhen 518055, China., Zhao C; Department of Biological and Agricultural Engineering, University of California, Davis, California 95616, United States., Mai X; Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, Davis, California 95616, United States., Emami S; Department of Food Science and Technology, University of California, Davis, California 95616, United States., Taha AY; Department of Food Science and Technology, University of California, Davis, California 95616, United States., Sun G; Department of Biological and Agricultural Engineering, University of California, Davis, California 95616, United States., Pan T; Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, Davis, California 95616, United States.; Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.; Shenzhen Engineering Laboratory of Single-Molecule Detection and Instrument Development, Shenzhen 518055, China.; Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou 215123, China.; Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230026, China. |
| Abstrakt: |
Enzyme-linked immunosorbent assays (ELISA), as one of the most used immunoassays, have been conducted ubiquitously in hospitals, research laboratories, etc. However, the conventional ELISA procedure is usually laborious, occupies bulky instruments, consumes lengthy operation time, and relies considerably on the skills of technicians, and such limitations call for innovations to develop a fully automated ELISA platform. In this paper, we have presented a system incorporating a robotic-microfluidic interface (RoMI) and a modular hybrid microfluidic chip that embeds a highly sensitive nanofibrous membrane, referred to as the Robotic ELISA, to achieve human-free sample-to-answer ELISA tests in a fully programmable and automated manner. It carries out multiple bioanalytical procedures to replace the manual steps involved in classic ELISA operations, including the pneumatically driven high-precision pipetting, efficient mixing and enrichment enabled by back-and-forth flows, washing, and integrated machine vision for colorimetric readout. The Robotic ELISA platform has achieved a low limit of detection of 0.1 ng/mL in the detection of a low sample volume (15 μL) of chloramphenicol within 20 min without human intervention, which is significantly faster than that of the conventional ELISA procedure. Benefiting from its modular design and automated operations, the Robotic ELISA platform has great potential to be deployed for a broad range of detections in various resource-limited settings or high-risk environments, where human involvement needs to be minimized while the testing timeliness, consistency, and sensitivity are all desired. |
