In vitro fibrinolytic activity of an enzyme purified from Bacillus amyloliquefaciens strain KJ10 isolated from soybean paste .

Autor: Rajaselvam J; Bioprocess Engineering Division, Smykon Biotech Pvt LtD, Nagercoil, Kanyakumari, Tamil Nadu 629201, India., Benit N; Department of Botany, Holy Cross College, Nagercoil, Kanyakumari District, Tamil Nadu 629 001, India., Alotaibi SS; Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Rathi MA; Department of Biochemistry, Sree Narayana Guru College, Coimbatore, Tamil Nadu 641 105, India., Srigopalram S; Application Specialist, Genurem Bisciences Tiruchirapalli, Tamil Nadu 620021,India., Biji GD; Department of Zoology, Nesamony Memorial Christian College, Marthandam, Kanyakumari, Tamil Nadu 629 165, India., Vijayaraghavan P; Bioprocess Engineering Division, Smykon Biotech Pvt LtD, Nagercoil, Kanyakumari, Tamil Nadu 629201, India.
Jazyk: angličtina
Zdroj: Saudi journal of biological sciences [Saudi J Biol Sci] 2021 Aug; Vol. 28 (8), pp. 4117-4123. Date of Electronic Publication: 2021 May 01.
DOI: 10.1016/j.sjbs.2021.04.061
Abstrakt: A fibrinolytic protease secreting producing Bacillus amyloliquefaciens strain KJ10 was initially screened from the fermented soybean. Maximum productivity was obtained in the culture medium after 40 h incubation, 34 °C incubation temperature at pH 8.0. Fibrinolytic protease production was enhanced in the culture medium with 1% sucrose (3712 ± 52 U/mL), 1% (w/v) yeast extract (3940 ± 28 U/mL) and 0.1% MgSO 4 (3687 ± 38 U/mL). Enzyme was purified up to 22.9-fold with 26%recovery after Q-Sepharose HP column chromatography. After three steps purification, enzyme activity was 1606U/mg and SDS-PAGE analysis revealed 29 kDa protein and enzyme band was detected by zymograpy. Enzyme was highly active at pH 8.0, at wide temperature ranges (40 °C - 55 °C) and was activated by Mn 2+ (102 ± 3.1%) and Mg 2+ (101.4 ± 2.9%) ions. The purified fibrinolytic enzyme was highly specific against N-Suc-Ala-Ala-Pro-Phe- p NA (189 mmol/min/mL) and clot lytic activity reached 28 ± 1.8% within 60 min in vitro . The purified fibrinolytic enzyme showed least erythrocytic lysis activity confirmed safety to prevent various health risks, including hemolytic anemia. Based on this study, administration of fibrinolytic enzyme from B. amyloliquefaciens strain KJ10 is safe for clinical applications.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2021 The Authors.)
Databáze: MEDLINE
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