Compact portable multiphoton microscopy reveals histopathological hallmarks of unprocessed lung tumor tissue in real time.

Autor: van Huizen LMG; Faculty of Science, Department of Physics, LaserLab Vrije Universiteit Amsterdam Amsterdam Netherlands., Radonic T; Department of Pathology Amsterdam Universities Medical Center/VU University Medical Center Amsterdam Netherlands., van Mourik F; Femto Diagnostics B.V. Uithoorn Netherlands., Seinstra D; Department of Pathology Amsterdam Universities Medical Center/VU University Medical Center Amsterdam Netherlands., Dickhoff C; Department of Surgery Amsterdam Universities Medical Center Amsterdam Netherlands., Daniels JMA; Department of Pulmonary Diseases Amsterdam Universities Medical Center Amsterdam Netherlands., Bahce I; Department of Pulmonary Diseases Amsterdam Universities Medical Center Amsterdam Netherlands., Annema JT; Department of Pulmonary Diseases Amsterdam Universities Medical Center Amsterdam Netherlands., Groot ML; Faculty of Science, Department of Physics, LaserLab Vrije Universiteit Amsterdam Amsterdam Netherlands.
Jazyk: angličtina
Zdroj: Translational biophotonics [Transl Biophotonics] 2020 Nov; Vol. 2 (4), pp. e202000009. Date of Electronic Publication: 2020 Aug 21.
DOI: 10.1002/tbio.202000009
Abstrakt: During lung cancer operations a rapid and reliable assessment of tumor tissue can reduce operation time and potentially improve patient outcomes. We show that third harmonic generation (THG), second harmonic generation (SHG) and two-photon excited autofluorescence (2PEF) microscopy reveals relevant, histopathological information within seconds in fresh unprocessed human lung samples. We used a compact, portable microscope and recorded images within 1 to 3 seconds using a power of 5 mW. The generated THG/SHG/2PEF images of tumorous and nontumorous tissues are compared with the corresponding standard histology images, to identify alveolar structures and histopathological hallmarks. Cellular structures (tumor cells, macrophages and lymphocytes) (THG), collagen (SHG) and elastin (2PEF) are differentiated and allowed for rapid identification of carcinoid with solid growth pattern, minimally enlarged monomorphic cell nuclei with salt-and-pepper chromatin pattern, and adenocarcinoma with lipidic and micropapillary growth patterns. THG/SHG/2PEF imaging is thus a promising tool for clinical intraoperative assessment of lung tumor tissue.
Competing Interests: Frank van Mourik and Marie Louise Groot declare to have, respectively, direct and indirect interest in Femto Diagnostics. Laura M. G. van Huizen received technical support from Femto Diagnostics. Femto Diagnostics was not involved in design of the study or analysis of the data.
(© 2020 The Authors. Translational Biophotonics published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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